Chen Zhu, Kang Zhen, Xiao En-Hua, Tong Min, Xiao Yu-Dong, Li Hua-Bing
Zhu Chen, Zhen Kang, En-Hua Xiao, Yu-Dong Xiao, Hua-Bing Li, Department of Radiology, the Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China.
World J Gastroenterol. 2015 Apr 28;21(16):4875-82. doi: 10.3748/wjg.v21.i16.4875.
To compare two different laparotomy methods for modeling rabbit VX2 hepatocarcinoma.
Thirty New Zealand rabbits were randomly divided into two groups: A and B. Group A was assigned a traditional laparotomy method (embedding tumor fragments directly into the liver with tweezers). Group B was subjected to an improved laparotomy method (injection of tumor fragments into the liver through a 15 G syringe needle). The operation time, incision length, incision infection rate, and mortality rate were compared between the two groups after laparotomy. Magnetic resonance imaging (MRI) was performed to evaluate tumor formation rates and the characteristics of the tumors 2 wk after laparotomy.
The mean operation times for the two groups (Group A vs Group B) were 23.2 ± 3.4 min vs 17.5 ± 2.9 min (P < 0.05); the incision length was 3.3 ± 0.5 cm vs 2.4 ± 0.6 cm (P < 0.05); and the mortality rate after 2 wk was 26.7% vs 0% (P < 0.05); all of these outcomes were significantly different between the two groups. The incision infection rates in the two groups were 6.7% vs 0% (P > 0.05), which were not significantly different. MRI performed after 2 weeks showed that the tumor formation rates in the two groups were 90.9% vs 93.3% (P > 0.05). These rates were not significantly different between the two groups. The celiac implantation rate and abdominal wall metastasis rate in the two groups were 36.4% vs 13.3% (P < 0.05) and 27.2% vs 6.7% (P < 0.05), respectively, which were significantly different between the two groups.
The tumor formation rates were not significantly different between the two methods for modeling rabbit VX2 hepatocarcinoma. However, the improved method is recommended because it has certain advantages.
比较两种不同的剖腹术方法用于建立兔VX2肝癌模型。
30只新西兰兔随机分为两组：A组和B组。A组采用传统剖腹术方法（用镊子将肿瘤碎片直接植入肝脏）。B组采用改良剖腹术方法（通过15G注射器针头将肿瘤碎片注入肝脏）。比较两组剖腹术后的手术时间、切口长度、切口感染率和死亡率。剖腹术后2周进行磁共振成像（MRI）以评估肿瘤形成率和肿瘤特征。
两组的平均手术时间（A组对B组）分别为23.2±3.4分钟对17.5±2.9分钟（P<0.05）；切口长度为3.3±0.5厘米对2.4±0.6厘米（P<0.05）；2周后的死亡率为26.7%对0%（P<0.05）；所有这些结果在两组之间均有显著差异。两组的切口感染率分别为6.7%对0%（P>0.05），无显著差异。2周后进行的MRI显示，两组的肿瘤形成率分别为90.9%对93.3%（P>0.05）。两组之间这些比率无显著差异。两组的腹腔种植率和腹壁转移率分别为36.4%对13.3%（P<0.05）和27.2%对6.7%（P<0.05），两组之间有显著差异。
两种建立兔VX2肝癌模型的方法在肿瘤形成率上无显著差异。然而，推荐改良方法，因为它具有一定优势。
