Chen J H, Lin Y C, Huang Y S, Chen T J, Lin W Y, Han K W
Department of Radiology, China Medical College and Hospital, Taichung, Taiwan.
Lab Anim. 2004 Jan;38(1):79-84. doi: 10.1258/00236770460734434.
Direct injection of VX2 cell suspension into the liver is simple and widely used. Implantation of a fragment of VX2 tumour into the liver using a surgical technique has also been developed in the last decade. In this study, we compared these two methods in order to find a better modality for establishing VX2 liver mass. Forty rabbits, each weighing 2.8-3.2 kg, were divided into two groups, 20 rabbits in each. In Group 1, a tumour cell suspension containing 1 x 10(6) cells in a volume of 0.1 ml, was injected slowly into the liver parenchyma using a 27-gauge needle during laparotomy. In Group 2, a 1 mm(3) fragment of VX2 carcinoma was inoculated into the sub-capsule of the left anterior lobe of the liver. In Group 1, three rabbits showed no tumour growth and 10 rabbits showed evidence of leakage and tumour seeding outside of the liver. In Group 2, all but one rabbit showed tumour growth and none showed evidence of tumour seeding. The leakage rates were 50% and 0% for Group 1 and Group 2, respectively. Overall, the success inoculation rate was 35% for Group 1 and 95% for Group 2. In conclusion, to create the VX2 liver tumour model in rabbits, direct implantation of VX2 tumour fragment into the liver achieved better results than injecting cell suspension of VX2 tumour into the liver.
将VX2细胞悬液直接注射到肝脏中操作简单且应用广泛。在过去十年中，也开发了使用外科技术将VX2肿瘤碎片植入肝脏的方法。在本研究中，我们比较了这两种方法，以找到建立VX2肝肿块的更好方式。40只体重2.8 - 3.2千克的兔子被分为两组，每组20只。在第1组中，在剖腹手术期间使用27号针头将含有1×10(6)个细胞、体积为0.1毫升的肿瘤细胞悬液缓慢注入肝实质。在第2组中，将1立方毫米的VX2癌碎片接种到肝脏左前叶的包膜下。在第1组中，3只兔子未出现肿瘤生长，10只兔子出现肝脏外渗漏和肿瘤种植迹象。在第2组中，除1只兔子外所有兔子均出现肿瘤生长，且无肿瘤种植迹象。第1组和第2组的渗漏率分别为50%和0%。总体而言，第1组的成功接种率为35%，第2组为95%。总之，为在兔子中创建VX2肝肿瘤模型，将VX2肿瘤碎片直接植入肝脏比将VX2肿瘤细胞悬液注入肝脏取得了更好的结果。
