Payame Noor University, Tehran Center Unit, Tehran, Iran.
Department of Biochemistry and Biophysics, Babol University of Medical Sciences, Babol, Iran.
Iran J Basic Med Sci. 2015 Mar;18(3):277-83.
To evaluate the effect of trinitroglycerin (TNG) as nitric oxide donor agent on serum copper (Cu) and zinc (Zn) levels and liver enzymes in BALB/c mice infected with Leishmania major (L. major) MRHO/IR/75/ER.
Inbred female mice were divided into three groups: healthy group (uninfected naive mice), control group (infected with L. major), and test group (L. major infected mice treated with TNG). TNG (200 µg/µl) was inoculated subcutaneously into the mice of the test group. Serum Cu and Zn levels and liver enzymes activities were then evaluated by atomic absorption spectrophometer and colorimetric methods, respectively.
Serum Cu levels were significantly higher in the test group than in the control and naive groups (P-value <0.05), while Zn levels were higher in the test group than in the control group with no significant difference. Serum glutamicoxaloacetic transaminase concentrations in the test group were significantly lower than those in other groups (P-value <0.05), while serum glutamate pyruvic transaminase concentrations were significantly higher in test compared with those in other groups (P-value <0.05). Moreover, alkaline phosphatase in the control and test groups were significantly lower than that in the naive group (P-value <0.05).
TNG treatment increased Zn and Cu levels and thus increased resistance to Leishmania because of the role of Zn and Cu; therefore, TNG therapy will be useful for treating cutaneous leishmania. In addition, the decrease of serum glutamicoxaloacetic transaminase activity can be an index of therapeutic process of TNG.
评估三硝酸甘油酯（TNG）作为一氧化氮供体对感染利什曼原虫（L. major）MRHO/IR/75/ER 的 BALB/c 小鼠血清铜（Cu）和锌（Zn）水平及肝酶的影响。
将雌性近交系小鼠分为三组：健康组（未感染的未感染幼稚鼠）、对照组（感染 L. major）和实验组（感染 L. major 并用 TNG 治疗的小鼠）。实验组皮下接种 TNG（200 µg/µl）。然后用原子吸收分光光度计和比色法分别评估血清 Cu 和 Zn 水平及肝酶活性。
实验组血清 Cu 水平明显高于对照组和健康组（P 值<0.05），而实验组 Zn 水平高于对照组但无统计学差异。实验组血清谷草转氨酶浓度明显低于其他组（P 值<0.05），而实验组血清谷氨酸丙酮酸转氨酶浓度明显高于其他组（P 值<0.05）。此外，对照组和实验组的碱性磷酸酶明显低于健康组（P 值<0.05）。
TNG 治疗增加了 Zn 和 Cu 水平，从而提高了对利什曼原虫的抵抗力，因为 Zn 和 Cu 的作用；因此，TNG 治疗将有助于治疗皮肤利什曼病。此外，血清谷氨酸草酰乙酸转氨酶活性的降低可能是 TNG 治疗过程的一个指标。
