枣(Zizyphus jujuba)水提物可减轻糖尿病周围神经病变体外模型中葡萄糖诱导的神经毒性。

Aqueous extract of Zizyphus jujuba fruit attenuates glucose induced neurotoxicity in an in vitro model of diabetic neuropathy.

机构信息

Department of Physiology, School of Veterinary Medicine, Lorestan University, Khorramabad, Iran.

Kerman Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran.

出版信息

Iran J Basic Med Sci. 2015 Mar;18(3):301-6.

OBJECTIVES

The neuroprotective effect of fruit aqueous extract of Ziziphus jujuba Lam on glucose-induced neurotoxicity in PC12 cells as an appropriate in vitro model of diabetic neuropathy was investigated.

MATERIALS AND METHODS

Cell viability was determined by the MTT assay. Cellular reactive oxygen species (ROS) generation was measured by DCFH-DA analysis. Cleaved caspase-3, a biochemical parameter of cellular apoptosis, was measured by western blot analysis.

RESULTS

Our data showed that a 4-fold elevation in glucose levels within the medium significantly reduced cell viability, increased intracellular ROS and caspase-3 activation in PC12 cells after 24 hr. Incubation of the high glucose medium cells with 300-μg/ml Z. jujuba fruit (ZJF) extract decreased the high glucose-induced cell toxicity and prevented caspase-3 activation and excited ROS generation.

CONCLUSION

Thus, we concluded that the aqueous extract of Z. jujuba protects against hyperglycaemia-induced cellular toxicity. This could be associated with the prevention of ROS generation and neural apoptosis. Moreover, the results suggest that the ZJF has a therapeutic potential to attenuate diabetes complications such as neuropathy.

目的

研究酸枣(Ziziphus jujuba Lam)果实水提物对葡萄糖诱导的 PC12 细胞神经毒性的神经保护作用，PC12 细胞作为糖尿病周围神经病变的合适体外模型。

材料和方法

通过 MTT 测定法测定细胞活力。通过 DCFH-DA 分析测定细胞活性氧（ROS）的产生。通过 Western blot 分析测定细胞凋亡的生化参数——裂解的 caspase-3。

结果

我们的数据表明，培养基中葡萄糖水平升高 4 倍会显著降低 PC12 细胞的活力，在 24 小时后增加细胞内 ROS 和 caspase-3 的激活。用 300-μg/ml 的酸枣(Z. jujuba)果实（ZJF）提取物孵育高葡萄糖培养基细胞可降低高葡萄糖诱导的细胞毒性，并可预防 caspase-3 激活和兴奋 ROS 的产生。