Sato Masaaki, Kawano Masako, Ohkura Masamichi, Gengyo-Ando Keiko, Nakai Junichi, Hayashi Yasunori
RIKEN Brain Science Institute, Wako, Saitama, Japan; PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama, Japan.
RIKEN Brain Science Institute, Wako, Saitama, Japan.
PLoS One. 2015 May 6;10(5):e0125354. doi: 10.1371/journal.pone.0125354. eCollection 2015.
The spatiotemporally controlled expression of G-CaMP fluorescent calcium indicator proteins can facilitate reliable imaging of brain circuit activity. Here, we generated a transgenic mouse line that expresses G-CaMP7 under a tetracycline response element. When crossed with a forebrain-specific tetracycline-controlled transactivator driver line, the mice expressed G-CaMP7 in defined cell populations in a tetracycline-controlled manner, notably in pyramidal neurons in layer 2/3 of the cortex and in the CA1 area of the hippocampus; this expression allowed for imaging of the in vivo activity of these circuits. This mouse line thus provides a useful genetic tool for controlled G-CaMP expression in vivo.
G-CaMP荧光钙指示剂蛋白的时空控制表达有助于对脑回路活动进行可靠成像。在此，我们构建了一个在四环素反应元件控制下表达G-CaMP7的转基因小鼠品系。当与一个前脑特异性四环素控制的反式激活因子驱动品系杂交时，这些小鼠以四环素控制的方式在特定细胞群体中表达G-CaMP7，特别是在皮质第2/3层的锥体神经元和海马体的CA1区；这种表达使得能够对这些回路的体内活动进行成像。因此，该小鼠品系为体内可控的G-CaMP表达提供了一种有用的遗传工具。
