Dai Yu, Wu Zhen, Zhou Qiheng, Zhao Qun, Li Ningning, Xie Shuguang, Liu Yong
State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.
Key Laboratory of Water and Sediment Sciences (Ministry of Education), College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.
Res Microbiol. 2015 Oct;166(8):655-63. doi: 10.1016/j.resmic.2015.07.012. Epub 2015 Aug 6.
Both ammonia-oxidizing archaea (AOA) and bacteria (AOB) can be involved in biotransformation of ammonia to nitrite in soil ecosystems. However, the distribution of AOA and AOB in plateau soils and influential factors remain largely unclear. In the present study, the activity, abundance and structure of ammonia oxidizers in different soils on the Yunnan Plateau were assessed using potential nitrification rates (PNRs), quantitative PCR assay and clone library analysis, respectively. Wide variation was found in both AOA and AOB communities in plateau soils. PNRs showed a significant positive correlation with AOB abundance. Both were determined by the ratio of organic carbon to nitrogen (C/N) and total phosphorous (TP). AOB could play a more important role in ammonia oxidation. AOB community diversity was likely affected by soil total nitrogen (TN) and total organic carbon (TOC) and was usually higher than AOA community diversity. Moreover, Nitrososphaera- and Nitrosospira-like organisms, respectively, were the dominant AOA and AOB in plateau soils. AOA community structure was likely shaped by TP and C/N, while AOB community structure was determined by pH.
氨氧化古菌（AOA）和细菌（AOB）都可参与土壤生态系统中氨向亚硝酸盐的生物转化过程。然而，AOA和AOB在高原土壤中的分布及其影响因素仍不清楚。在本研究中，分别采用潜在硝化速率（PNR）、定量PCR分析和克隆文库分析，对云南高原不同土壤中氨氧化菌的活性、丰度和结构进行了评估。研究发现，高原土壤中AOA和AOB群落存在广泛差异。PNR与AOB丰度呈显著正相关。二者均由有机碳氮比（C/N）和总磷（TP）决定。AOB在氨氧化过程中可能发挥更重要的作用。AOB群落多样性可能受土壤全氮（TN）和总有机碳（TOC）影响，且通常高于AOA群落多样性。此外，类硝化球菌属和类亚硝化螺菌属生物分别是高原土壤中占主导地位的AOA和AOB。AOA群落结构可能受TP和C/N影响，而AOB群落结构则由pH决定。
