Kim Min Jee, Choi Sei-Woong, Kim Iksoo
College of Agriculture & Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea; Email: unknown.
Department of Environmental Education, Mokpo National University, Muan, Jeonnam, 534-729, Republic of Korea; Email: unknown.
Zootaxa. 2015 Apr 10;3946(3):374-86. doi: 10.11646/zootaxa.3946.3.5.
Saturnia (Rinaca) jonasii Butler, 1877 is distributed in Japan, including Tsushima Island and Taiwan, whereas S. boisduvalii Eversmann, 1846 is distributed in northern areas, such as China, Russia, and South Korea. In the present study we found that the specimens from Mt. Hallasan on Jejudo, a southern remote offshore island, were S. jonasii, rather than S. boisduvalii based on morphology, DNA barcode, and nuclear elongation factor 1 alpha (EF-1α) sequences. The major morphological differences between the two species included the shape of wing pattern elements of fore- and hindwings and male and female genitalia. A DNA barcode analysis of the sequences of the Jejudo specimens and S. boisduvalii, along with those of Saturnia species obtained from a public database showed a minimum sequence divergence of 4.26% (28 bp). A phylogenetic analysis also showed clustering of the Jejudo specimens with S. jonasii, separating S. boisduvalii (Bayesian posterior probability = 0.99). The EF-1α-based sequence and phylogenetic analyses of the two species from Jejudo Island and the Korean mainland showed the uniqueness of the Jejudo specimens from S. boisduvalii collected on the Korean mainland, indicating distribution of S. jonasii on Jejudo Island in South Korea, instead of S. boisduvalii.
乔氏大蚕蛾（Saturnia (Rinaca) jonasii Butler，1877）分布于日本，包括对马岛和台湾，而 Boisduval 大蚕蛾（S. boisduvalii Eversmann，1846）分布于中国、俄罗斯和韩国等北方地区。在本研究中，我们发现，根据形态学、DNA 条形码和核延伸因子 1α（EF - 1α）序列，来自韩国南部偏远近海岛屿济州岛汉拿山的标本是乔氏大蚕蛾，而非 Boisduval 大蚕蛾。这两个物种之间的主要形态差异包括前翅和后翅的翅纹元素形状以及雄性和雌性生殖器。对济州岛标本和 Boisduval 大蚕蛾的序列进行的 DNA 条形码分析，以及从公共数据库获得的大蚕蛾物种的序列分析显示，最小序列差异为 ～4.26%（28 个碱基对）。系统发育分析还显示，济州岛标本与乔氏大蚕蛾聚类，与 Boisduval 大蚕蛾分离（贝叶斯后验概率 = 0.99）。基于 EF - 1α的序列分析和对济州岛和韩国大陆的两个物种的系统发育分析表明，济州岛标本与在韩国大陆采集的 Boisduval 大蚕蛾不同，这表明韩国济州岛分布的是乔氏大蚕蛾，而非 Boisduval 大蚕蛾。
