Suksaard Paweena, Duangmal Kannika, Srivibool Rattanaporn, Xie Qingyi, Hong Kui, Pathom-Aree Wasu
Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Center for Advanced Studies in Tropical Natural Resources, NRU-KU, Kasetsart University, Chatuchak, Bangkok, Thailand.
Int J Syst Evol Microbiol. 2015 Aug;65(8):2569-2573. doi: 10.1099/ijs.0.000303. Epub 2015 May 6.
An aerobic, Gram-stain-positive actinomycete, designated strain 3SM4-07T, was characterized using a polyphasic taxonomic approach. The strain produced branching mycelium which fragmented into short or elongated rods. The whole-cell hydrolysates contained ll-2,6-diaminopimelic acid as the diagnostic diamino acid, with glucose and ribose as the main sugars. The predominant cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.The predominant menaquinone was MK-9(H4). Phospholipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and phosphatidylinositol mannoside. Mycolic acids were absent. The DNA G+C content was 72.3 mol%. Strain 3SM4-07T formed a phylogenetic line within the genus Jiangella and its 16S rRNA gene sequence was related most closely to Jiangella alkaliphila D8-87T (99.0% similarity), Jiangella muralis 15-Je-017T (98.8%), Jiangella alba YIM 61503T (98.6%) and Jiangella gansuensis YIM 002T (98.6%). However, mean DNA-DNA hybridization values revealed that strain 3SM4-07T differed from the closest species previously described in this genus. Data from phenotypic, chemotaxonomic and molecular analyses between strain 3SM4-07T and recognized species of the genus Jiangella indicate that strain 3SM4-07T is a representative of a novel species of the genus Jiangella, for which the name Jiangella mangrovi sp. nov. is proposed. The type strain is 3SM4-07T ( = BCC 60398T = NBRC 109648T).
采用多相分类方法对一株需氧、革兰氏染色阳性的放线菌3SM4-07T进行了特征描述。该菌株产生分支菌丝体，可断裂成短杆状或长杆状。全细胞水解产物含有ll-2,6-二氨基庚二酸作为诊断性二氨基酸，主要糖类为葡萄糖和核糖。主要的细胞脂肪酸为anteiso-C15 : 0、iso-C15 : 0和iso-C16 : 0。主要的甲基萘醌为MK-9(H4)。磷脂由双磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱、磷脂酰肌醇和磷脂酰肌醇甘露糖苷组成。不存在分枝菌酸。DNA G+C含量为72.3 mol%。菌株3SM4-07T在Jiangella属内形成一个系统发育分支，其16S rRNA基因序列与嗜碱Jiangella菌D8-87T（相似度99.0%）、壁生Jiangella菌15-Je-017T（98.8%）、白色Jiangella菌YIM 61503T（98.6%）和甘肃Jiangella菌YIM 002T（98.6%）关系最为密切。然而，平均DNA-DNA杂交值表明菌株3SM4-07T与该属先前描述的最接近物种不同。菌株3SM4-07T与Jiangella属已确认物种之间的表型、化学分类和分子分析数据表明，菌株3SM4-07T是Jiangella属一个新物种的代表，为此提出新名称红树林Jiangella菌（Jiangella mangrovi sp. nov.）。模式菌株为3SM4-07T（=BCC 60398T=NBRC 109648T）。
