Jiangella rhizosphaerae sp. nov., an actinomycete isolated from the rhizosphere soil of wheat (Triticum aestivum L.).

A novel actinomycete, designated strain NEAU-YY265, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) and characterized using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NEAU-YY265 belonged to the genus Jiangella and exhibited 16S rRNA gene sequence similarities of 99.1 and 99.1 % to Jiangella mangrovi 3SM4-07 and Jiangella alkaliphila D8-87. The chemotaxonomic properties of strain NEAU-YY265 were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid; the whole-cell hydrolysates contained glucose, ribose and rhamnose; MK-9(H4) and MK-9(H2) were the predominant menaquinones. The novel strain produced branching mycelium which fragmented into short or elongated rods. The major fatty acids (>5 %) were anteiso-C15 : 0, anteiso-C17 : 0, C17 : 1ω8c, iso-C16 : 0, C17 : 0 and iso-C15 : 0. The DNA G+C content was 72.6 mol%. Strain NEAU-YY265 was phenotypically distinct from recognized Jiangella species and shown to belong to a separate genomic species based on DNA-DNA hybridization results. Thus, strain NEAU-YY265 is considered to represent a novel species of the genus Jiangella, for which the name Jiangella rhizosphaerae sp. nov. is proposed. The type strain is NEAU-YY265 (=JCM 32551=CGMCC 4.7475).