Nyangale Edna P, Farmer Sean, Cash Howard A, Keller David, Chernoff David, Gibson Glenn R
Food Microbial Sciences Unit, Department of Food and Nutritional Sciences, The University of Reading, Reading, United Kingdom;
Ganeden Biotech, Mayfield Heights, OH; and.
J Nutr. 2015 Jul;145(7):1446-52. doi: 10.3945/jn.114.199802. Epub 2015 May 6.
Advancing age is linked to a decrease in beneficial bacteria such as Bifidobacterium spp. and reduced aspects of innate immune function.
We investigated whether daily consumption of a probiotic [Bacillus coagulans GBI-30, 6086 (BC30); GanedenBC(30)] could improve immune function and gut function in men and women aged 65-80 y, using a double-blind, placebo-controlled crossover design.
Thirty-six volunteers were recruited and randomly assigned to receive either a placebo (microcrystalline cellulose) or the probiotic BC30 (1 × 10(9) colony-forming units/capsule). Volunteers consumed 1 treatment capsule per day for 28 d, followed by a 21-d washout period before switching to the other treatment. Blood and fecal samples were collected at the beginning and end of each treatment period. Fecal samples were used to enumerate bacterial groups and concentrations of calprotectin. Peripheral blood mononuclear cells (PBMCs) were extracted from whole blood to assess natural killer cell activity and lipopolysaccharide (LPS)-stimulated cytokine production. C-reactive protein concentrations were measured in plasma.
Consumption of BC30 significantly increased populations of Faecalibacterium prausnitzii by 0.1 log10 cells/mL more than during consumption of the placebo (P = 0.03), whereas populations of Bacillus spp. increased significantly by 0.5 log10 cells/mL from baseline in volunteers who consumed BC30 (P = 0.007). LPS-stimulated PBMCs showed a 0.2 ng/mL increase in the anti-inflammatory cytokine IL-10 28 d after consumption of BC30 (P < 0.05), whereas the placebo did not affect IL-10, and no overall difference was found in the effect of the treatments.
Daily consumption of BC30 by adults aged 65-80 y can increase beneficial groups of bacteria in the human gut and potentially increase production of anti-inflammatory cytokines. This study shows the potential benefits of a probiotic to improve dysbiosis via modulation of the microbiota in older persons.
年龄增长与有益细菌（如双歧杆菌属）数量减少以及先天免疫功能的某些方面下降有关。
我们采用双盲、安慰剂对照交叉设计，研究每日食用益生菌[凝结芽孢杆菌GBI-30, 6086（BC30）；GanedenBC(30)]是否能改善65至80岁男女的免疫功能和肠道功能。
招募了36名志愿者，随机分配接受安慰剂（微晶纤维素）或益生菌BC30（1×10⁹ 菌落形成单位/胶囊）。志愿者每天服用1粒治疗胶囊，持续28天，随后有21天的洗脱期，之后换用另一种治疗。在每个治疗期开始和结束时采集血液和粪便样本。粪便样本用于计数细菌菌群和钙卫蛋白浓度。从全血中提取外周血单核细胞（PBMC）以评估自然杀伤细胞活性和脂多糖（LPS）刺激的细胞因子产生。测量血浆中C反应蛋白浓度。
食用BC30后，普拉梭菌的数量比食用安慰剂期间显著增加0.1 log₁₀ 细胞/毫升（P = 0.03），而芽孢杆菌属的数量在食用BC30的志愿者中从基线显著增加0.5 log₁₀ 细胞/毫升（P = 0.007）。食用BC30 28天后，LPS刺激的PBMC显示抗炎细胞因子IL-10增加0.2纳克/毫升（P < 0.05），而安慰剂对IL-10没有影响，且各治疗效果无总体差异。
65至80岁成年人每日食用BC30可增加人体肠道中的有益细菌菌群，并可能增加抗炎细胞因子的产生。本研究显示了益生菌通过调节老年人微生物群改善生态失调的潜在益处。
