Gepner Yftach, Hoffman Jay R, Shemesh Elad, Stout Jeffrey R, Church David D, Varanoske Alyssa N, Zelicha Hila, Shelef Ilan, Chen Yacov, Frankel Hagai, Ostfeld Ishay
Sport and Exercise Science, Institute of Exercise Physiology and Wellness, University of Central Florida, Orlando, Florida.
Sport and Exercise Science, Institute of Exercise Physiology and Wellness, University of Central Florida, Orlando, Florida;
J Appl Physiol (1985). 2017 Jul 1;123(1):11-18. doi: 10.1152/japplphysiol.01116.2016. Epub 2017 Apr 13.
The purpose of this study was to compare the coadministration of the probiotic GBI-30, 6086 (BC30) with β-hydroxy-β-methylbutyrate (HMB) calcium (CaHMB) to CaHMB alone on inflammatory response and muscle integrity during 40 days of intense military training. Soldiers were randomly assigned to one of two groups: CaHMB with BC30 (CaHMBBC30; = 9) or CaHMB with placebo (CaHMBPL, = 9). A third group of participants served as a control (CTL; = 8). During the first 28 days soldiers were garrisoned on base and participated in the same training tasks. During the final 2 wk soldiers navigated 25-30 km per night in difficult terrain carrying ~35 kg of equipment. All assessments (blood draws and diffusion tensor imaging to assess muscle integrity) were conducted before and ~12 h after final supplement consumption. Analysis of covariance was used to analyze all blood and muscle measures. Significant attenuations were noted in IL-1β, IL-2, IL-6, CX3CL1, and TNF-α for both CaHMBBC30 and CaHMBPL compared with CTL. Plasma IL-10 concentrations were significantly attenuated for CaHMBBC30 compared with CTL only. A significant decrease in apparent diffusion coefficients was also observed for CaHMBBC30 compared with CaHMBPL. Results provide further evidence that HMB supplementation may attenuate the inflammatory response to intense training and that the combination of the probiotic BC30 with CaHMB may be more beneficial than CaHMB alone in maintaining muscle integrity during intense military training. β-Hydroxy-β-methylbutyrate (HMB) in its free acid form was reported to attenuate inflammation and maintain muscle integrity during military training. However, this formulation was difficult to maintain in the field. In this investigation, soldiers ingested HMB calcium (CaHMB) with (BC30) or CaHMB alone during 40 days of training. Results indicated that CaHMB attenuated the inflammatory response and that BC30 combined with CaHMB may be more beneficial than CaHMB alone in maintaining muscle integrity during intense military training.
本研究的目的是比较益生菌GBI-30、6086（BC30）与β-羟基-β-甲基丁酸（HMB）钙（CaHMB）联合使用和单独使用CaHMB对高强度军事训练40天期间炎症反应和肌肉完整性的影响。士兵被随机分为两组：CaHMB与BC30联合组（CaHMBBC30；n = 9）或CaHMB与安慰剂联合组（CaHMBPL，n = 9）。第三组参与者作为对照组（CTL；n = 8）。在前28天，士兵们驻扎在基地并参与相同的训练任务。在最后2周，士兵们每晚在困难地形中携带约35公斤装备行进25 - 30公里。所有评估（采血和扩散张量成像以评估肌肉完整性）均在最后一次补充剂服用前和约12小时后进行。采用协方差分析来分析所有血液和肌肉指标。与CTL相比，CaHMBBC30和CaHMBPL组的IL-1β、IL-2、IL-6、CX3CL1和TNF-α均有显著降低。仅与CTL相比，CaHMBBC30组的血浆IL-10浓度显著降低。与CaHMBPL相比，CaHMBBC30组的表观扩散系数也有显著下降。结果提供了进一步的证据，表明补充HMB可能减轻对高强度训练的炎症反应，并且在高强度军事训练期间，益生菌BC30与CaHMB联合使用可能比单独使用CaHMB在维持肌肉完整性方面更有益。据报道，游离酸形式的β-羟基-β-甲基丁酸（HMB）在军事训练期间可减轻炎症并维持肌肉完整性。然而，这种制剂在野外难以保存。在本研究中，士兵们在40天的训练期间摄入了含（BC30）的HMB钙（CaHMB）或单独摄入CaHMB。结果表明，CaHMB减轻了炎症反应，并且在高强度军事训练期间，BC30与CaHMB联合使用可能比单独使用CaHMB在维持肌肉完整性方面更有益。
