三丁基锡和多环芳烃对日本鳗鲡（Anguilla japonica）CYP1A、芳烃受体和卵黄蛋白原基因表达的影响
Effect of TBT and PAHs on CYP1A, AhR and Vitellogenin Gene Expression in the Japanese Eel, Anguilla japonica.
作者信息
Choi Min Seop, Kwon Se Ryun, Choi Seong Hee, Kwon Hyuk Chu
机构信息
Dept. of Aquatic Life Medical Science, Sun Moon University, Asan 336-708, Korea.
Dept. of Food Science, Sun Moon University, Asan 336-708, Korea.
出版信息
Dev Reprod. 2012 Dec;16(4):289-94. doi: 10.12717/DR.2012.16.4.289.
Gene expressions of cytochrome P4501A (CYP1A), aryl hydrocarbon receptor (AhR) and vitellogenin (Vg) by endocrine disruptors, benzo[α]pyrene (B[a]P) and tributyltin (TBT) were examined in cultured eel hepatocytes which were isolated from eels treated previously with B[a]P (10 mg/kg) or estradiol-17β (20 mg/kg) in vivo, and the relationship between CYP1A, AhR and Vg genes were studied. When the cultured eel hepatocytes were treated with B[a]P (10(-6)-10(-5) M) the gene expressions of CYP1A and AhR were enhanced in a concentration-dependent manner. However, when treated with TBT (10(-9)-10(-5) M) the gene expressions of CYP1A and AhR were suppressed at high concentrations (10(-6)-10(-5) M), while having no effects at low concentrations (10(-9)-10(-7) M). Gene expression of Vg was also suppressed by TBT in a concentration-dependent manner in cultured eel hepatocytes which was previously treated in vivo with estradiol-17β.
在内分泌干扰物苯并[a]芘（B[a]P）和三丁基锡（TBT）作用下，对从体内预先用B[a]P（10毫克/千克）或17β-雌二醇（20毫克/千克）处理过的鳗鱼分离得到的培养鳗鱼肝细胞中细胞色素P4501A（CYP1A）、芳烃受体（AhR）和卵黄蛋白原（Vg）的基因表达进行了检测，并研究了CYP1A、AhR和Vg基因之间的关系。当用B[a]P（10^(-6)-10^(-5) M）处理培养的鳗鱼肝细胞时，CYP1A和AhR的基因表达呈浓度依赖性增强。然而，当用TBT（10^(-9)-10^(-5) M）处理时，高浓度（10^(-6)-10^(-5) M）下CYP1A和AhR的基因表达受到抑制，而低浓度（10^(-9)-10^(-7) M）时则无影响。在体内预先用17β-雌二醇处理过的培养鳗鱼肝细胞中，Vg的基因表达也受到TBT的浓度依赖性抑制。
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