Kim Dae-Hyun, Yoon Jong-Man
Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University, Gunsan 573-701, Republic of Korea.
Dev Reprod. 2014 Jun;18(2):89-98. doi: 10.12717/DR.2014.18.2.089.
The twenty-one individuals of Meretrix lusoria were secured from Gunsan, Shinan and Yeonggwang on the coast of the Yellow Sea and the southern sea in the Korean Peninsula, respectively. Amplification of a single COI fragment (720 bp) was imagined, and no apparent size differences were observed in amplified fragments between Meretrix lusoria and M. petechialis individuals. The size of the DNA fragments also varied excitedly, from 200 to 1,600 bp. The oligonucleotides primer BION-08 produced the least loci (a total of 17), with an average of 2.43 in the Gunsan population, in comparison to the other primers used. Remarkably, the primer BION-13 detected 42 shared loci by the three populations, major and/or minor fragments of sizes 200 bp and 400 bp, respectively, which were identical in all samples. The dendrogram gained by the seven oligonucleotides primers highlight three genetic clusters: cluster 1 (GUNSAN 01 ~ GUNSAN 07), cluster 2 (SHINAN 08 ~ SHINAN 14) and cluster 3 (YEONGGWANG 15 ~ YEONGGWANG 21). The longest genetic distance among the twenty-one Meretrix lusoria individuals that displayed significant molecular differences was between individuals GUNSAN no. 01 and SHINAN no. 14 (genetic distance = 0.574). Comparatively, individuals of SHINAN population were fairly closely related to that of YEONGGWANG population. In this study, PCR analysis has discovered significant genetic distances between two white clam population pairs (P<0.05).
21只泥蚶分别采自韩国半岛黄海沿岸的群山、新安和全罗南道海南郡。设想扩增单个COI片段（720 bp），泥蚶个体与菲律宾蛤仔个体之间扩增片段未观察到明显大小差异。DNA片段大小也剧烈变化，从200到1600 bp。与使用的其他引物相比，寡核苷酸引物BION - 08产生的位点最少（总共17个），群山种群平均为2.43个。值得注意的是，引物BION - 13检测到三个种群共有的42个位点，分别为大小200 bp和400 bp的主要和/或次要片段，在所有样本中均相同。由七个寡核苷酸引物得到的树状图突出显示了三个遗传簇：簇1（群山01 ~ 群山07）、簇2（新安08 ~ 新安14）和簇3（海南15 ~ 海南21）。在显示出显著分子差异 的21只泥蚶个体中，最长的遗传距离出现在群山01号个体和新安14号个体之间（遗传距离 = 0.574）。相比之下，新安种群个体与海南种群个体关系相当密切。在本研究中，PCR分析发现两个白蛤种群对之间存在显著的遗传距离（P<0.05）。
