Yoon Jong-Man
Dept. of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University, Gunsan 54150, Korea.
Dev Reprod. 2019 Dec;23(4):377-384. doi: 10.12717/DR.2019.23.4.377. Epub 2019 Dec 31.
Genomic DNA extracted from representatives of two populations, Gunsan and Chinese, of spp. was amplified using PCR with several primers. The band-sharing (BS) value between individuals no. 05 from the Gunsan population and no. 22 from the Chinese population was 0.206, which was the lowest recognized value. Oligonucleotides primer OPC-04 revealed 44 unique loci, which distinguished the Chinese population. Primer OPB-17 allowed the discovery of 22 loci shared by the two populations, which were present in all samples. Based on the average BS results, individuals from the Gunsan population demonstrated lower BS values (0.661±0.012) than did those from the Chinese population (0.788±0.014; <0.05). The shortest genetic distance (GD) displaying a noteworthy molecular difference was between individuals CHINESE no. 12 and no. 13 (GD=0.027). Individual no. 06 from the Gunsan population was most distantly related to CHINESE no. 22 (GD=0.703). A group tree of the two populations was constructed by UPGMA Euclidean GD analysis based on a total of 543 fragments generated using six primers. The explicit markers recognized in this study will be used for genetic analysis, as well as to evaluate the species security and proliferation of echiuran individuals in intertidal regions of the Korean Peninsula.
从多毛纲动物的群山种群和中国种群的代表中提取基因组DNA，使用几种引物通过聚合酶链式反应（PCR）进行扩增。群山种群的05号个体与中国种群的22号个体之间的条带共享（BS）值为0.206，这是所识别到的最低值。寡核苷酸引物OPC - 04揭示了44个独特位点，这些位点区分了中国种群。引物OPB - 17发现了两个种群共有的22个位点，这些位点存在于所有样本中。基于平均BS结果，群山种群个体的BS值（0.661±0.012）低于中国种群个体（0.788±0.014；P<0.05）。显示出显著分子差异的最短遗传距离（GD）存在于中国种群的12号和13号个体之间（GD = 0.027）。群山种群的06号个体与中国种群的22号个体亲缘关系最远（GD = 0.703）。基于使用六种引物产生的总共543个片段，通过UPGMA欧几里得GD分析构建了两个种群的聚类树。本研究中识别出的明确标记将用于遗传分析，以及评估朝鲜半岛潮间带多毛纲动物个体的物种安全性和增殖情况。
