甜叶菊（Stevia rebaudiana (Bert.) Bertoni）根和叶中低聚果糖的结构与聚合度
Structure and degree of polymerisation of fructooligosaccharides present in roots and leaves of Stevia rebaudiana (Bert.) Bertoni.
作者信息
de Oliveira Arildo José Braz, Gonçalves Regina Aparecida Correia, Chierrito Talita Perez Cantuaria, Dos Santos Marcelo Müller, de Souza Lauro Mera, Gorin Philip Albert James, Sassaki Guilherme Lanzi, Iacomini Marcello
机构信息
Departamento de Farmácia, Universidade Estadual de Maringá, CEP 87.020-900, Maringá, PR, Brazil.
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CEP 81.531-980, CP 19046, Curitiba, PR, Brazil.
出版信息
Food Chem. 2011 Nov 15;129(2):305-311. doi: 10.1016/j.foodchem.2011.04.057. Epub 2011 May 1.
Fructooligosaccharides have been isolated from roots and leaves of Stevia rebaudiana, by hot aqueous extraction, followed by precipitation with ethanol. Their structure has been determined using methylation and NMR analysis, MALDI-TOF, and ESI-MS. Fructooligosaccharides contained almost exclusively (2→1)-linked β-fructofuranosyl, with terminal α-glucopyranosyl and β-fructofuranosyl units. MALDI-TOF and ESI-MS analyses showed the wide range of degree of polymerisation (DP) present in various extracts. From roots and leaves, three different fractions gave profiles of homologous series, with DPs ranging up to 17 with MALDI-TOF and 19 using ESI-MS. These inulin-type fructooligosaccharides were the major component of extracts from S. rebaudiana roots and significant components from the leaves.
低聚果糖已从甜叶菊的根和叶中分离出来，采用热水萃取法，随后用乙醇沉淀。其结构已通过甲基化和核磁共振分析、基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）和电喷雾电离质谱（ESI-MS）确定。低聚果糖几乎只含有（2→1）连接的β-呋喃果糖基，带有末端α-吡喃葡萄糖基和β-呋喃果糖基单元。MALDI-TOF和ESI-MS分析表明，各种提取物中存在广泛的聚合度（DP）范围。从根和叶中，三个不同的组分给出了同系物系列的图谱，MALDI-TOF测得的DP高达17，ESI-MS测得的DP高达19。这些菊粉型低聚果糖是甜叶菊根提取物的主要成分，也是叶提取物的重要成分。
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