Chanter N, Goodwin R F, Rutter J M
Agricultural and Food Research Council, Institute for Animal Health, Compton, Newbury, Berkshire.
Res Vet Sci. 1989 Nov;47(3):355-8.
The efficacy of detecting toxigenic Pasteurella multocida from nasal swabs of slaughtered and live pigs was assessed. The isolation of toxigenic P multocida from nasal cavities of slaughtered bacon pigs from two herds with atrophic rhinitis was reduced by immersion in the hot water tank by 25 per cent and 75 per cent. Individual sows from one of the infected herds were repeatedly swabbed to find the best method of isolating toxigenic P multocida. Toxigenic P multocida were isolated from 50 per cent of cotton swabs inoculated on to selective medium the same day. After 24 hours in the post, 45 per cent of cotton swabs placed in transport medium, 38 per cent of alginate swabs dissolved in transport medium and inoculated into mice, and 36 per cent of the dissolved swabs inoculated directly on to selective medium yielded toxigenic P multocida. These bacteria were isolated from only 25 per cent of cotton swabs held in transport medium at 10 degrees C for 48 hours to simulate prolonged postage times; from slaughtered pigs a similar reduction in isolation was seen with swabs kept for 24 or 48 hours. The reduced isolation caused by a delay before culture was associated with an overgrowth of other flora. The development of this flora was prevented by storage of swabs at 4 degrees C in the laboratory or by the use of cool boxes for postage.
评估了从屠宰猪和活猪的鼻拭子中检测产毒素多杀性巴氏杆菌的效果。将来自两个患有萎缩性鼻炎猪群的屠宰腌肉型猪鼻腔中产毒素多杀性巴氏杆菌的分离率，通过浸入热水箱分别降低了25%和75%。对其中一个感染猪群的母猪个体反复进行拭子采样，以找到分离产毒素多杀性巴氏杆菌的最佳方法。当天接种到选择性培养基上的棉拭子中有50%分离出产毒素多杀性巴氏杆菌。邮寄24小时后，置于运输培养基中的棉拭子有45%、溶解于运输培养基并接种到小鼠体内的藻酸盐拭子有38%、直接接种到选择性培养基上的溶解拭子有36%分离出产毒素多杀性巴氏杆菌。在10摄氏度下于运输培养基中保存48小时以模拟延长邮寄时间的棉拭子中，只有25%分离出这些细菌；对于屠宰猪，保存24小时或48小时的拭子也出现了类似的分离率降低情况。培养前的延迟导致分离率降低与其他菌群过度生长有关。通过在实验室中于4摄氏度保存拭子或使用冷藏箱邮寄可防止这种菌群的生长。
