Kawamoto E, Sawada T, Maruyama T
Animal Research Center, Tokyo Medical College, Shinjuku, Japan.
J Clin Microbiol. 1997 Aug;35(8):1948-51. doi: 10.1128/jcm.35.8.1948-1951.1997.
A suitable medium for the transport of Pasteurella multocida in nasal specimens from rabbits was investigated by using pure cultures of the organism and nasal swabs from infected rabbits. First, the ability of eight transport media to preserve the viabilities of P. multocida strains isolated from rabbits was studied. Cary-Blair medium and Leibovitz medium no. 15 (L-15) were found to be superior to the other six media tested, enabling survival of the organism for more than 14 days at room temperature. Second, the survival of P. multocida in nasal specimens was evaluated on both Cary-Blair medium and L-15. The recovery rate of the organism from these two media was more than 80 to 90% during 4 days of storage and decreased gradually with increasing preservation time. There were no significant differences (P > 0.05) in recovery rates of the organism between Cary-Blair medium and L-15. On the basis of these results, we recommend the use of Cary-Blair medium for the transport of P. multocida in rabbit nasal specimens because of the ease of transport of nasal swabs by mail.
通过使用该生物体的纯培养物和来自感染兔子的鼻拭子，对一种适合在兔子鼻标本中运输多杀巴斯德菌的培养基进行了研究。首先，研究了八种运输培养基保存从兔子分离的多杀巴斯德菌菌株活力的能力。发现 Cary-Blair 培养基和 Leibovitz 15 号培养基（L-15）优于其他六种测试培养基，能使该生物体在室温下存活超过 14 天。其次，在 Cary-Blair 培养基和 L-15 上评估了多杀巴斯德菌在鼻标本中的存活情况。在储存 4 天期间，从这两种培养基中分离出该生物体的回收率超过 80%至 90%，并随着保存时间的增加而逐渐下降。Cary-Blair 培养基和 L-15 之间该生物体的回收率没有显著差异（P>0.05）。基于这些结果，我们推荐使用 Cary-Blair 培养基在兔子鼻标本中运输多杀巴斯德菌，因为鼻拭子便于邮寄运输。
