采用液相色谱-串联质谱法测定牛尿中的兽用抗生素。
Determination of veterinary antibiotics in bovine urine by liquid chromatography-tandem mass spectrometry.
机构信息
Department of Veterinary Science and Public Health, University of Milan, Via Celoria 10, 20133 Milan, Italy.
Department of Health, Animal Science and Food Safety, University of Milan, Via Celoria 10, 20133 Milan, Italy.
出版信息
Food Chem. 2015 Oct 15;185:7-15. doi: 10.1016/j.foodchem.2015.03.098. Epub 2015 Mar 28.
A follow-up of antibiotics (tetracyclines, fluoroquinolones, cephalosporins, penicillins and amphenicols) in the bovine urine is important for two reasons: to understand if they are still present in organism, and whether their occurrence in urine might be considered as an environmental risk. A validated HPLC-MS/MS method (Decision 2002/657/EC) for antibiotics determination in bovine urine was developed. CCα and CCβ were in the range of 0.58-0.83 and 0.55-1.1 ng mL(-1), respectively. Recoveries were 92-108%, with inter-day repeatability below 12%. Analysis of bovine urine revealed frequent presence of tetracyclines, which was related with animal's age. The cause, most presumably, might be found in different therapeutic protocols applied for veal calves and young bulls enrolled in this study. Most abundant was oxytetracycline with highest level in veal calves (1718 ng mL(-1)) vs. young bulls (2.8 ng mL(-1)). Our results indicate the necessity of antibiotics monitoring in bovine urine before animals undergo further processing in the food industry.
牛尿中抗生素（四环素类、氟喹诺酮类、头孢菌素类、青霉素类和安普霉素）的跟踪监测非常重要，原因有二：一是了解抗生素是否仍存在于动物体内，二是尿液中抗生素的存在是否可能被视为环境风险。本研究建立了一种用于牛尿中抗生素检测的经验证的 HPLC-MS/MS 方法（Decision 2002/657/EC）。CCα 和 CCβ 的范围分别为 0.58-0.83 和 0.55-1.1ng/mL。回收率为 92-108%，日内重复性低于 12%。牛尿分析显示，四环素类抗生素经常存在，这与动物的年龄有关。最有可能的原因是，在本研究中，犊牛和小公牛采用了不同的治疗方案。检测到的最丰富的是土霉素，在犊牛（1718ng/mL）中的含量最高，而在小公牛（2.8ng/mL）中的含量较低。我们的研究结果表明，在动物进入食品工业进一步加工之前，有必要对牛尿中的抗生素进行监测。
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