Dyková I, Tyml T
Department of Botany and Zoology, Faculty of Science, Masaryk University, Brno, Czech Republic.
Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.
J Fish Dis. 2016 May;39(5):539-46. doi: 10.1111/jfd.12384. Epub 2015 May 7.
The case study targeted to determine the aetiology of nodular gill disease (NGD) of farmed rainbow trout. The methods included microscopical examination of gill material in fresh, culturing of isolated organisms, histology, transmission electron microscopy and molecular biology identification. The results revealed an intravital colonization of fish gills by the testate amoeba Rhogostoma minus Belar, 1921. Rhogostoma infection was found in all fish examined microscopically (15/15); in contrast, naked amoebae related to fully developed NGD lesions were found in minority of these fish (5/15). They belonged to four genera, Acanthamoeba, Vermamoeba, Naegleria and Vannella. Results presented in this study contribute to the mosaic of findings that contrary to amoebic gill disease of marine fish turn attention to the possibility of the heterogeneous, multi-amoeba-species and multifactorial aetiology of NGD.
该案例研究旨在确定养殖虹鳟鱼结节性鳃病（NGD）的病因。方法包括对新鲜鳃组织进行显微镜检查、分离生物的培养、组织学、透射电子显微镜检查和分子生物学鉴定。结果显示，1921年发现的有壳变形虫微小罗氏变形虫（Rhogostoma minus Belar）在鱼鳃内有活体定殖。在所有接受显微镜检查的鱼（15/15）中均发现了罗氏变形虫感染；相比之下，在这些鱼中的少数（5/15）发现了与完全发展的NGD病变相关的裸露变形虫。它们属于四个属，棘阿米巴属（Acanthamoeba）、维氏阿米巴属（Vermamoeba）、耐格里属（Naegleria）和范氏阿米巴属（Vannella）。本研究提出的结果有助于完善研究结果拼图，与海水鱼的阿米巴鳃病不同，该结果将注意力转向了NGD病因的异质性、多变形虫物种和多因素性的可能性。
