Lin Jia-Horng, Chen Chih-Kuang, Wen Shih-Peng, Lou Ching-Wen
Laboratory of Fiber Application and Manufacturing, Department of Fiber and Composite Materials, Feng Chia University, Taichung 40724, Taiwan, ROC; School of Chinese Medicine, China Medical University, Taichung 40402, Taiwan, ROC; Department of Fashion Design, Asia University, Taichung 41354, Taiwan, ROC.
The Polymeric Biomaterials Lab, Department of Fiber and Composite Materials, Feng Chia University, Taichung 40724, Taiwan, ROC.
Mater Sci Eng C Mater Biol Appl. 2015;52:111-20. doi: 10.1016/j.msec.2015.03.034. Epub 2015 Mar 24.
Bone scaffolds require a three-dimensional structure, high porosity, interconnected pores, adequate mechanical strengths, and non-toxicity. A high porosity is incongruent with mechanical strengths. Therefore, this study combines a braiding method and microsphere solution to create bone scaffolds with a high porosity and sufficient mechanical strengths. First, poly-L-lactide (PLLA) plied yarns are braided into 5-, 10-, 15-, 20-, and 25-layer hollow braids, and then thermally treated at 165 °C for various durations. Next, sodium alginate (SA) microspheres, cross-linked with CaCl2 solution with various concentrations, are combined with PLLA porous braided bone scaffolds to form PLLA/SA/CS microsphere hybrid scaffolds, which are then observed for surface observation, and tested for porosity, water contact angle, compressive strength, MTT assay, bioactivity, alkaline phosphatase (ALP) assay, cell attachment, and statistical analyses. The test results show that the layer amount of the bone scaffold is proportional to the compressive strength. With the same number of layers, the compressive strength is inversely proportional to the concentration of the CaCl2 solution. The results of surface observation, porosity, and water contact angle tests show that PLLA/SA/CS microsphere hybrid scaffolds possess a high porosity and good hydrophilicity; as a result, the braiding manufacture and the bonding technique effectively solve the confliction between porosity and mechanical strength. The concentration of CaCl2 does not pertain to cell activity and ALP results, exemplified by good cell attachment on bone scaffolds for each specification.
骨支架需要三维结构、高孔隙率、相互连通的孔隙、足够的机械强度和无毒性。高孔隙率与机械强度不相容。因此，本研究结合编织方法和微球溶液来制备具有高孔隙率和足够机械强度的骨支架。首先，将聚-L-丙交酯（PLLA）合股纱编织成5层、10层、15层、20层和25层的中空编织物，然后在165℃下进行不同时长的热处理。接下来，将与不同浓度CaCl2溶液交联的海藻酸钠（SA）微球与PLLA多孔编织骨支架结合，形成PLLA/SA/CS微球混合支架，然后对其进行表面观察、孔隙率、水接触角、抗压强度、MTT法、生物活性、碱性磷酸酶（ALP）测定、细胞附着测试及统计分析。测试结果表明，骨支架的层数与抗压强度成正比。在层数相同的情况下，抗压强度与CaCl2溶液的浓度成反比。表面观察、孔隙率和水接触角测试结果表明，PLLA/SA/CS微球混合支架具有高孔隙率和良好的亲水性；因此，编织制造和粘结技术有效地解决了孔隙率和机械强度之间的矛盾。CaCl2的浓度与细胞活性和ALP结果无关，各规格骨支架上细胞附着良好即为例证。
