Lou Ching-Wen, Huang Chien-Lin, Chen Chih-Kuang, Liu Chi-Fan, Wen Shih-Peng, Lin Jia-Horng
Institute of Biomedical Engineering and Materials Science, Central Taiwan University of Science and Technology, Taichung 40601, Taiwan.
Department of Fiber and Composite Materials, Feng Chia University, Taichung City 40724, Taiwan.
Materials (Basel). 2015 Dec 14;8(12):8768-8779. doi: 10.3390/ma8125488.
In order to solve the incompatibility between high porosity and mechanical properties, this study fabricates bone scaffolds by combining braids and sodium alginate (SA) membranes. Polyethylene terephthalate (PET) plied yarns are braided into hollow, porous three dimensional (3D) PET braids, which are then immersed in SA solution, followed by cross-linking with calcium chloride (CaCl₂) and drying, to form PET bone scaffolds. Next, SA membranes are rolled and then inserted into the braids to form the spiral and porous PET/SA bone scaffolds. Samples are finally evaluated for surface observation, porosity, water contact angle, compressive strength, and MTT assay. The test results show that the PET bone scaffolds and PET/SA bone scaffolds both have good hydrophilicity. An increasing number of layers and an increasing CaCl₂ concentration cause the messy, loose surface structure to become neat and compact, which, in turn, decreases the porosity and increases the compressive strength. The MTT assay results show that the cell viability of differing SA membranes is beyond 100%, indicating that the PET/SA bone scaffolds containing SA membranes are biocompatible for cell attachment and proliferation.
为了解决高孔隙率与机械性能之间的不相容性问题，本研究通过将编织物与海藻酸钠（SA）膜相结合来制备骨支架。将聚对苯二甲酸乙二酯（PET）合股线编织成中空、多孔的三维（3D）PET编织物，然后将其浸入SA溶液中，接着与氯化钙（CaCl₂）交联并干燥，以形成PET骨支架。接下来，将SA膜卷起并插入编织物中，以形成螺旋状且多孔的PET/SA骨支架。最后对样品进行表面观察、孔隙率、水接触角、抗压强度和MTT测定。测试结果表明，PET骨支架和PET/SA骨支架均具有良好的亲水性。层数增加和CaCl₂浓度升高会使杂乱、松散的表面结构变得整齐致密，进而降低孔隙率并提高抗压强度。MTT测定结果表明，不同SA膜的细胞活力超过100%，这表明含有SA膜的PET/SA骨支架对细胞附着和增殖具有生物相容性。
