白细胞介素-16基因多态性与膝关节骨关节炎风险
Genetic polymorphisms of interleukin-16 and risk of knee osteoarthritis.
作者信息
Luo Shi-Xing, Li Shan, Zhang Xue-Hui, Zhang Jun-Jing, Long Guang-Hua, Dong Gui-Fu, Su Wei, Deng Yan, Liu Yanqiong, Zhao Jin-Min, Qin Xue
机构信息
Department of Orthopedic Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China; Department of Trauma Orthopedics, Ninth Affiliated Hospital of Guangxi Medical University, Beihai, Guangxi, China.
Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
出版信息
PLoS One. 2015 May 8;10(5):e0123442. doi: 10.1371/journal.pone.0123442. eCollection 2015.
BACKGROUND
Interleukin-16 (IL-16), a pleiotropic cytokine, plays a fundamental role in inflammatory diseases. This study investigates the association between IL-16 polymorphisms and the risk of knee osteoarthritis (OA) in a Chinese population.
METHODS
The IL-16 rs11556218, rs4072111, and rs4778889 polymorphisms were determined in 150 knee OA cases and 147 healthy controls through polymerase chain reaction-restriction fragment length polymorphism.
RESULTS
The results suggested that the variants in IL-16 gene rs11556218 site were associated with a decreased knee OA risk after adjusting for age, sex, BMI, and smoking and drinking status (TG vs. TT: OR, 0.69; 95% CI, 0.53-0.89; P = 0.006; GG vs. TT: OR, 0.64; 95% CI, 0.45-0.90; P = 0.042; dominant model: OR, 0.68; 95% CI, 0.29-0.87; P = 0.002; G vs. T allele: OR, 0.77; 95% CI, 0.66-0.90; P = 0.003). Similarly, subjects bearing the rs4072111 variant genotypes and alleles also had a lower susceptibility to knee OA compared with those bearing the wild-type (CT vs. CC: OR, 0.66; 95% CI, 0.53-0.83; P = 0.002; TT vs. CC: OR, 0.57; 95% CI, 0.40-0.82; P = 0.027; dominant model: OR, 0.65; 95%, CI 0.52-0.80; P <0.001; T vs. C allele: OR, 0.69; 95% CI, 0.58-0.81; P <0.001). Further, the C allele and the combined genotype (CC+CT) of rs4778889 were associated with a slightly decreased risk of knee OA. In addition, we found two high-risk haplotypes: TTT (OR, 3.70) and GCC (OR, 6.22). Finally, serum IL-16 levels of knee OA patients were significantly higher than those of controls (P = 0.001).
CONCLUSIONS
Despite the small sample size, this is the first study suggesting IL-16 gene polymorphisms to be associated with the risk of knee OA.
背景
白细胞介素-16（IL-16）是一种多效性细胞因子，在炎症性疾病中起重要作用。本研究调查了中国人群中IL-16基因多态性与膝关节骨关节炎（OA）风险之间的关联。
方法
通过聚合酶链反应-限制性片段长度多态性方法，对150例膝关节OA患者和147例健康对照者的IL-16基因rs11556218、rs4072111和rs4778889多态性进行检测。
结果
结果表明，在调整年龄、性别、体重指数以及吸烟和饮酒状况后，IL-16基因rs11556218位点的变异与膝关节OA风险降低相关（TG与TT相比：比值比[OR]，0.69；95%置信区间[CI]，0.53 - 0.89；P = 0.006；GG与TT相比：OR，0.64；95% CI，0.45 - 0.90；P = 0.042；显性模型：OR，0.68；95% CI，0.29 - 0.87；P = 0.002；G与T等位基因相比：OR，0.77；95% CI，0.66 - 0.90；P = 0.003）。同样，携带rs4072111变异基因型和等位基因的受试者与携带野生型的受试者相比，对膝关节OA的易感性也较低（CT与CC相比：OR，0.66；95% CI，0.53 - 0.83；P = 0.002；TT与CC相比：OR，0.57；95% CI，0.40 - 0.82；P = 0.027；显性模型：OR，0.65；95% CI，0.52 - 0.80；P <0.001；T与C等位基因相比：OR，0.69；95% CI，0.58 - 0.81；P <0.001）。此外，rs4778889的C等位基因和组合基因型（CC + CT）与膝关节OA风险略有降低相关。另外发现了两种高危单倍型：TTT（OR，3.70）和GCC（OR，6.22）。最后，膝关节OA患者的血清IL-16水平显著高于对照组（P = 0.001）。
结论
尽管样本量较小，但这是第一项表明IL-16基因多态性与膝关节OA风险相关的研究。
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