Post-Graduation Program in Biosciences and Physiopathology, Department of Clinical Analysis and Biomedicine, and Laboratory of Immunogenetics, State University of Maringá, Maringá, Paraná, Brazil.
Department of Basic Health Sciences, State University of Maringá, Maringá, Paraná, Brazil.
PLoS One. 2020 Sep 11;15(9):e0239101. doi: 10.1371/journal.pone.0239101. eCollection 2020.
Periodontitis (PD) is a chronic inflammatory process resulting from the relationship of the immune response with the components in dental plaque. Cytokines and their genetic polymorphisms seem to be involved in the immunopathogenesis of this disease. This study aimed to evaluate the correlation of IL16 polymorphism with PD. A case-control study was conducted in a sample of individuals from southern Brazil. The genotyping of IL16, rs11556218 T>G, rs4072111 C>T e rs4778889 T>C, was performed using the PCR-RFLP methodology. The serum level of IL-16 was determined using an IL-16 ELISA kit for humans. SNPStats and OpenEpi software and Wilcoxon's U test were used to perform statistical analysis. IL16 rs11556218 polymorphism was significantly associated to PD in nonsmoking patients: individuals with G/G genotype were less likely to develop PD compared to the T/T genotype (OR = 0.10; Pc = 0.019, codominant model). In addition, the TTT haplotype was associated with a high risk for PD (OR = 2.45; P = 0.01). A low IL-16 serum level was observed among individuals with PD when compared to controls (P = 0.027). Thus, the IL16 rs16556218 polymorphism and the serum levels of IL-16 were associated with periodontitis in a Brazilian sample, and this was influenced by environmental factors such as smoking.
牙周炎（PD）是一种慢性炎症过程，是由免疫反应与牙菌斑成分之间的关系引起的。细胞因子及其遗传多态性似乎参与了这种疾病的免疫发病机制。本研究旨在评估 IL16 多态性与 PD 的相关性。在巴西南部的一个个体样本中进行了病例对照研究。使用 PCR-RFLP 方法对 IL16、rs11556218T>G、rs4072111C>T 和 rs4778889T>C 进行基因分型。使用人类 IL-16 ELISA 试剂盒测定血清中 IL-16 的水平。使用 SNPStats 和 OpenEpi 软件和 Wilcoxon U 检验进行统计分析。IL16 rs11556218 多态性与非吸烟患者的 PD 显著相关：与 T/T 基因型相比，G/G 基因型的个体发生 PD 的可能性较小（OR=0.10；Pc=0.019，显性模型）。此外，TTT 单倍型与 PD 的高风险相关（OR=2.45；P=0.01）。与对照组相比，PD 患者的血清 IL-16 水平较低（P=0.027）。因此，IL16 rs16556218 多态性和 IL-16 的血清水平与巴西样本中的牙周炎相关，这受到吸烟等环境因素的影响。
