Gonul Oyku, Aydin Hikmet Hakan, Kalmis Erbil, Kayalar Husniye, Ozkaya Ali Burak, Atay Sevcan, Ak Handan
Department of Medical Biochemistry, Ege University, Bornova, Izmir, Turkey.
Ministry of Science, Industry and Technology, Manisa, Turkey.
Int J Med Mushrooms. 2015;17(3):231-9. doi: 10.1615/intjmedmushrooms.v17.i3.30.
Ganoderma lucidum is a medicinal higher Basidiomycetes mushroom that exerts anticancer effects through several different mechanisms. This study investigated the effects of G. lucidum on the telomerase activity and microRNA (miRNA) profiles of MCF-7 cells. According to the cytotoxicity results, the G. lucidum ether extract exhibits the highest cytotoxic potency; therefore it was chosen for the subsequent telomerase activity assay and miRNA profiling. The telomerase activity observed in the cells treated with a half-maximal inhibitory concentration of G. lucidum ether extract (100 µg/mL in dimethyl sulfoxide) was 32.2% lower than that of the control cells treated with 1% dimethyl sulfoxide. Among 1066 miRNAs, the most downregulated miRNA was hsa-miR-27a* (4.469-fold), and the most upregulated miRNA was hsa-miR-1285 (10.462-fold). A database search revealed the predicted miRNAs that target the catalytic subunit of the telomerase enzyme telomerase reverse transcriptase, and only miR-3687 (upregulated 2.153-fold) and miR-1207-5p (upregulated 2.895-fold) were changed by at least 2-fold. The miRNA profile changes demonstrated in this study provide a data set regarding their effects on the pathways that regulate telomerase activity in MCF-7 breast cancer cells treated with G. lucidum. These data should aid the development of novel cancer treatment strategies.
灵芝是一种具有药用价值的高等担子菌蘑菇，可通过多种不同机制发挥抗癌作用。本研究调查了灵芝对MCF-7细胞端粒酶活性和微小RNA（miRNA）谱的影响。根据细胞毒性结果，灵芝乙醚提取物表现出最高的细胞毒性效力；因此，它被选用于后续的端粒酶活性测定和miRNA分析。在用灵芝乙醚提取物的半数抑制浓度（在二甲基亚砜中为100μg/mL）处理的细胞中观察到的端粒酶活性比用1%二甲基亚砜处理的对照细胞低32.2%。在1066种miRNA中，下调最多的miRNA是hsa-miR-27a*（4.469倍），上调最多的miRNA是hsa-miR-1285（10.462倍）。数据库搜索揭示了靶向端粒酶逆转录酶催化亚基的预测miRNA，只有miR-3687（上调2.153倍）和miR-1207-5p（上调2.895倍）的变化至少为2倍。本研究中展示的miRNA谱变化提供了一组关于它们对用灵芝处理的MCF-7乳腺癌细胞中端粒酶活性调节途径影响的数据。这些数据应有助于开发新的癌症治疗策略。
