Li Zhipeng, Wright André-Denis G, Liu Hanlu, Fan Zhongyuan, Yang Fuhe, Zhang Zhigang, Li Guangyu
Jilin Provincial Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, China.
School of Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, Arizona, United States of America.
PLoS One. 2015 May 8;10(5):e0123481. doi: 10.1371/journal.pone.0123481. eCollection 2015.
High throughput sequencing was used to examine the rumen microbiota of sika deer fed high (OLH) and low concentration (OLL) of tannin rich oak leaves. The results showed that Prevotella spp. were the most dominant bacteria. The most predominant methanogens were the members of the order Methanoplasmatales. The dominant rumen protozoa were Entodinium longinucleatum, Eudiplodinium maggii, and Epidinium caudatum, and the fungal communities were mostly represented by Piromyces spp. Moreover, the relative abundance of Pseudobutyrivibrio spp. (P=0.026), unidentified bacteria (P=0.028), and Prevotella spp. (P=0.022) was lower in the OLH group than in the OLL group. The concentration of propionate in the OLH group was greater than in the OLL group (P=0.006). Patterns of relationships showed that methanogens belonging to the order Methanoplasmatales were negatively correlated with Treponema spp., Ent. Longinucleatum, and acetate. Methanosphaera stadtmanae was positively correlated to propionate, while Methanobrevibacter ruminantium was negatively associated with Methanobrevibacter thaueri and Methanobrevibacter millerae. Tannins altered the rumen microbes and fermentation patterns. However, the response of the entire rumen microbiota and the relationship between rumen microorganisms and the fermentation parameters were not fully understood.
采用高通量测序技术检测了饲喂高浓度（OLH）和低浓度（OLL）富含单宁栎树叶的梅花鹿瘤胃微生物群。结果表明，普雷沃氏菌属是最主要的细菌。最主要的产甲烷菌是甲烷微菌目成员。主要的瘤胃原生动物是长核内毛虫、马氏双毛虫和尾形双毛虫，真菌群落主要由梨形霉属代表。此外，OLH组中假丁酸弧菌属（P=0.026）、未鉴定细菌（P=0.028）和普雷沃氏菌属（P=0.022）的相对丰度低于OLL组。OLH组中丙酸浓度高于OLL组（P=0.006）。关系模式表明，属于甲烷微菌目的产甲烷菌与密螺旋体属、长核内毛虫和乙酸呈负相关。史氏甲烷球形菌与丙酸呈正相关，而反刍甲烷短杆菌与陶氏甲烷短杆菌和米勒氏甲烷短杆菌呈负相关。单宁改变了瘤胃微生物和发酵模式。然而，整个瘤胃微生物群的反应以及瘤胃微生物与发酵参数之间的关系尚未完全明了。
