Longo Valentina, Lana Alessandro, Bottero Maria Teresa, Zolla Lello
Department of Ecological and Biological Sciences, University of Tuscia, Largo dell'Università snc, 01100 Viterbo, Italy.
Department of Veterinary Sciences, University of Turin, via Leonardo da Vinci, 44, 10095 Grugliasco, Italy.
J Proteomics. 2015 Jul 1;125:29-40. doi: 10.1016/j.jprot.2015.04.023. Epub 2015 May 6.
Meat derives from a muscle that undergoes a great number of biochemical and physiological changes. The anoxic condition established from the moment of animal sacrifice forces muscle cells to a sort of reaction, resulting in methodical programmed cell death to avoid necrosis. The duality autophagy and/or apoptosis is at the center of the scientific debate about the biological processes driving the muscle to meat conversion. Here we report an omic time course overview carried on proteome, phosphoproteome and metabolome of Piedmontese longissimus thoracis muscle searching for clues helping us to extricate through the dilemma. The survey depicts a progressive physiological impairing and our evidences push towards the apoptotic behavior: the proteomic time course trend of annexin A2, RKIP, HSPB6, αB crystalline, adenylate kinase, DJ-1 and 31kDa actin fragment; the 0-1day increased phosphorylation of myosin 2 and synaptopodin and the metabolomic time course trend of key metabolic indicators, like GSH/GSSG ratio, taurine and nitrotyrosine. The employed techniques provide strong indications about the likely apoptotic behavior of aging meat in muscle-to-meat conversion process.
Our work underlines compelling evidences of the apoptotic behavior of Piedmontese beef muscle cells undergoing the muscle-to-meat process, whereas no autophagic clues are inferred from this omic investigation.
肉类源自经历大量生化和生理变化的肌肉。从动物屠宰之时起建立的缺氧状态迫使肌肉细胞产生一种反应，导致有条不紊的程序性细胞死亡以避免坏死。自噬和/或凋亡的二元性是关于驱动肌肉向肉类转化的生物学过程的科学辩论的核心。在此，我们报告了一项对皮埃蒙特胸最长肌的蛋白质组、磷酸蛋白质组和代谢组进行的组学时间进程概述，以寻找帮助我们摆脱困境的线索。该调查描绘了一种渐进性的生理损害，我们的证据倾向于凋亡行为：膜联蛋白A2、RKIP、HSPB6、αB晶状体蛋白、腺苷酸激酶、DJ-1和31kDa肌动蛋白片段的蛋白质组时间进程趋势；肌球蛋白2和突触足蛋白在0-1天磷酸化增加，以及关键代谢指标如谷胱甘肽/氧化型谷胱甘肽比值、牛磺酸和硝基酪氨酸的代谢组时间进程趋势。所采用的技术为老化肉类在肌肉向肉类转化过程中可能的凋亡行为提供了有力线索。
我们的工作强调了皮埃蒙特牛肉肌肉细胞在肌肉向肉类转化过程中凋亡行为的有力证据，而从这项组学研究中未推断出自噬线索。
