LBME du CNRS, France; Laboratoire de Biologie Moleculaire Eucaryote, Université de Toulouse, 118 route de Narbonne, F-31000 Toulouse, France.
Laboratory for Theoretical Physics of Condensed Matter UMR7600, Sorbonne University, UPMC, 75005 Paris, France; Groupement de recherche Architecture et Dynamique Nucléaire (GDR ADN), France.
Curr Opin Cell Biol. 2015 Jun;34:54-60. doi: 10.1016/j.ceb.2015.04.004. Epub 2015 May 15.
Nuclear organization can impact on all aspects of the genome life cycle. This organization is thoroughly investigated by advanced imaging and chromosome conformation capture techniques, providing considerable amount of datasets describing the spatial organization of chromosomes. In this review, we will focus on polymer models to describe chromosome statics and dynamics in the yeast Saccharomyces cerevisiae. We suggest that the equilibrium configuration of a polymer chain tethered at both ends and placed in a confined volume is consistent with the current literature, implying that local chromatin interactions play a secondary role in yeast nuclear organization. Future challenges are to reach an integrated multi-scale description of yeast chromosome organization, which is crucially needed to improve our understanding of the regulation of genomic transaction.
核组织可以影响基因组生命周期的各个方面。这种组织通过先进的成像和染色体构象捕获技术进行了深入研究，提供了大量描述染色体空间组织的数据集。在这篇综述中，我们将重点介绍聚合物模型来描述酵母酿酒酵母中的染色体静态和动态。我们建议，两端被束缚在受限体积中的聚合物链的平衡构型与当前文献一致，这意味着局部染色质相互作用在酵母核组织中起着次要作用。未来的挑战是达到酵母染色体组织的综合多尺度描述，这对于提高我们对基因组交易调控的理解至关重要。
