Raineri Jesica, Wang Songhu, Peleg Zvi, Blumwald Eduardo, Chan Raquel Lia
Instituto de Agrobiotecnología del Litoral (CONICET-UNL), Colectora ruta 168 km 0, Paraje El Pozo, 3000, Santa Fe, Argentina.
Plant Mol Biol. 2015 Jul;88(4-5):401-13. doi: 10.1007/s11103-015-0329-7. Epub 2015 May 9.
OsWRKY47 is a divergent rice transcription factor belonging to the group II of the WRKY family. A transcriptomic analysis of the drought response of transgenic rice plants expressing P SARK ::IPT, validated by qPCR, indicated that OsWRKY47 expression was induced under drought stress in P SARK ::IPT plants. A PCR-assisted site selection assay (SELEX) of recombinant OsWRKY47 protein showed that the preferred sequence bound in vitro is (G/T)TTGACT. Bioinformatics analyses identified a number of gene targets of OsWRKY47; among these two genes encode a Calmodulin binding protein and a Cys-rich secretory protein. Using Oswrk47 knockout mutants and transgenic rice overexpressing OsWRKY47 we show that the transcription of these putative targets were regulated by OsWRKY47. Phenotypic analysis carried out with transgenic rice plants showed that Oswrky47 mutants displayed higher sensitivity to drought and reduced yield, while plants overexpressing OsWRKY47 were more tolerant.
OsWRKY47是一种属于WRKY家族第二组的不同寻常的水稻转录因子。对表达P SARK::IPT的转基因水稻植株干旱响应的转录组分析（经qPCR验证）表明，在干旱胁迫下，P SARK::IPT植株中OsWRKY47的表达被诱导。重组OsWRKY47蛋白的PCR辅助位点选择分析（SELEX）表明，体外结合的优选序列是(G/T)TTGACT。生物信息学分析确定了OsWRKY47的许多基因靶点；其中两个基因编码一种钙调蛋白结合蛋白和一种富含半胱氨酸的分泌蛋白。使用Oswrk47基因敲除突变体和过表达OsWRKY47的转基因水稻，我们发现这些推定靶点的转录受OsWRKY47调控。对转基因水稻植株进行的表型分析表明，Oswrky47突变体对干旱表现出更高的敏感性且产量降低，而过表达OsWRKY47的植株更耐旱。
