de Castro Barra Patrícia Mendonça, Sabarense Céphora Maria, Alvarenga Marcelo Bonnet, de Sousa Rafael Arromba, de Oliveira Marcone Augusto Leal
Chemistry Department, Institute of Exact Sciences, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
Nutrition Department, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
Biol Trace Elem Res. 2015 Dec;168(2):441-6. doi: 10.1007/s12011-015-0359-7. Epub 2015 May 10.
The purpose of this work was to measure the amounts of selected mineral elements (sodium, calcium, iron, selenium, magnesium, zinc, copper, and manganese) in the liver of Wistar rats and evaluate possible correlations between the levels of these minerals and the lipid metabolism in the studied animals. Three experimental groups each containing six Wistar rats were designed. Each group was fed a different diet. The control group was fed a diet prepared with fresh soybean oil and named control group--CG. The second group (named experimental group B--EGB) and third group (named experimental group C--EGC) were fed a diet containing soybean oil that had been used to fry different foods for four or ten cycles, respectively. The mineral elements in Wistar rat livers were measured by inductively coupled plasma optical emission spectrometry (ICP OES). Only the elements calcium and selenium differed significantly between the control and experimental groups. There was a significant reduction of 33% for Ca and 41% for Se in the EGB in comparison to the control group. The reduction in mineral concentration, especially Se, is the result of interactions with fatty acid metabolism. The animals in the EGC exhibited more intracytoplasmic accumulation of fat and more intense vasodilatation, in relation to the other groups. Collectively, evidence hereby collected suggests that impaired dietary lipid quality in otherwise balanced diets can reduce hepatic Se levels and potentially harm liver function.
这项工作的目的是测量Wistar大鼠肝脏中选定的矿物质元素（钠、钙、铁、硒、镁、锌、铜和锰）的含量，并评估这些矿物质水平与所研究动物脂质代谢之间可能存在的相关性。设计了三个实验组，每组包含六只Wistar大鼠。每组喂食不同的饮食。对照组喂食用新鲜大豆油配制的饮食，称为对照组——CG。第二组（称为实验组B——EGB）和第三组（称为实验组C——EGC）分别喂食含有已用于煎炸不同食物四个或十个周期的大豆油的饮食。通过电感耦合等离子体发射光谱法（ICP OES）测量Wistar大鼠肝脏中的矿物质元素。对照组和实验组之间仅钙和硒元素存在显著差异。与对照组相比，EGB组中钙含量显著降低了33%，硒含量降低了41%。矿物质浓度的降低，尤其是硒，是与脂肪酸代谢相互作用的结果。与其他组相比，EGC组的动物表现出更多的胞浆内脂肪积累和更强烈的血管舒张。总体而言，此处收集的证据表明，在其他方面均衡的饮食中，膳食脂质质量受损会降低肝脏硒水平，并可能损害肝功能。
