BRD School of Biosciences, Vadtal Road, Satellite Campus, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar 388 120, Anand, Gujarat, India.
Bioresour Technol. 2015 Aug;190:219-26. doi: 10.1016/j.biortech.2015.04.090. Epub 2015 Apr 29.
The functionality and stability of phycobiliproteins (PBPs) phycoerythrin (PE), phycocyanin (PC) and allophycocyanin (APC) were investigated under various temperatures, pHs and oxidative stressors. All PBPs were thermostable up to 4-40°C; however, their concentration decreased rapidly at 60-80°C. The maximum stability of all PBPs was in the pH range 6.0-7.0. Decrease in PBPs content was found under high acidic (pH 2-4) and alkaline conditions (pH 8-12). The oxidizing agent (0.1-0.6%) showed the least effect on the stability of PBPs; however, 0.8-1.0% H2O2 caused significant loss of PBPs. Contrary to PE, PC and APC was more susceptible to an oxidizing agent. The chromophore associated with α- and β-subunit of PBPs and thus, their functionality (fluorescence) was severely affected under high temperature (60-80°C), and oxidizing agent, as well as low (2-4) and high (8-12) pH. Contrary to PC and APC, functionality of PE was surprisingly maintained even at pHs 6-12 and under oxidative stress.
研究了不同温度、pH 值和氧化应激条件下藻胆蛋白（PBPs）别藻蓝蛋白（PE）、藻蓝蛋白（PC）和藻红蛋白（APC）的功能和稳定性。所有 PBPs 在 4-40°C 下都是热稳定的；然而，它们的浓度在 60-80°C 时迅速下降。所有 PBPs 的最大稳定性在 pH 值 6.0-7.0 范围内。在高酸性（pH 2-4）和碱性条件（pH 8-12）下发现 PBPs 含量下降。氧化剂（0.1-0.6%）对 PBPs 稳定性的影响最小；然而，0.8-1.0%的 H2O2 会导致 PBPs 大量损失。与 PE 相反，PC 和 APC 对氧化剂更敏感。与 PBPs 的α-和β-亚基相关的生色团，因此，它们的功能（荧光）在高温（60-80°C）和氧化剂以及低（2-4）和高（8-12）pH 值下受到严重影响。与 PC 和 APC 相反，PE 的功能即使在 pH 值为 6-12 且在氧化应激下也出人意料地得以维持。
