Golijanin Ranko, Kujundžić Bojan, Milosavljević Zoran, Milovanović Dragan R, Andjelković Zlatibor, Obrenović Miroslav, Nikolić Radivoje
Vojnosanit Pregl. 2015 Mar;72(3):219-24. doi: 10.2298/vsp130627076g.
BACKGROUND/AIM: Periodontal disease affects gingival tissue and supporting apparatus of the teeth leading to its decay. The aim of this study was to highlight and precisely determine his- tological changes in the gum tissue.
Gingival biopsy samples from 53 healthy and parodontopathy-affected patients were used. Clinical staging of the disease was performed. Tissue specimens were fixed and routinely processed. Sections, 5 μm thin, were stained with hematoxylin and eosin, histochemical Van-Gieson for the collagen content, Spicer method for mast-cells and immunochemical method with anti-CD68 and anti-CD38 for the labelling of the macrophages and plasma-cells. Morphometric analysis was performed by a M42 test system.
While the disease advanced, collagen and fibroblast volume density decreased almost twice in the severe cases compared to the control ones, but a significant variation was observed within the investigated groups. The mast-cell number increased nearly two times, while the macrophage content was up to three times higher in severe parodontopathy than in healthy gingival tissue. However, the relative proportion of these cells stayed around 6% in all cases. Plasma-cells had the most prominent increase in the number (over 8 times) compared to the control, but again, a variation within investigated groups was very high.
Gingival tissue destruction caused by inflammatory process leads to significant changes in collagen density and population of resident connective tissue cells. Although inflammatory cells dominated with the disease advancing, a high variation within the same investigated groups suggests fluctuation of the pathological process.
背景/目的：牙周病会影响牙龈组织和牙齿的支持结构，导致牙齿腐烂。本研究的目的是突出并精确确定牙龈组织的组织学变化。
使用了来自53名健康和患有牙周病患者的牙龈活检样本。对疾病进行了临床分期。组织标本进行固定并常规处理。制作5微米厚的切片，用苏木精和伊红染色、用组织化学范吉森法检测胶原蛋白含量、用斯派塞法检测肥大细胞，并用抗CD68和抗CD38免疫化学方法标记巨噬细胞和浆细胞。通过M42测试系统进行形态计量分析。
随着疾病进展，与对照组相比，严重病例中的胶原蛋白和成纤维细胞体积密度几乎降低了两倍，但在研究组中观察到显著差异。肥大细胞数量增加了近两倍，而在严重牙周病中巨噬细胞含量比健康牙龈组织高多达三倍。然而，在所有情况下这些细胞的相对比例保持在6%左右。与对照组相比，浆细胞数量增加最为显著（超过8倍），但同样，研究组内部的差异非常大。
炎症过程导致的牙龈组织破坏会引起胶原蛋白密度和常驻结缔组织细胞数量的显著变化。尽管随着疾病进展炎症细胞占主导，但同一研究组内的高度差异表明病理过程存在波动。
