[Effects of conjugated linoleic acid and exercise on RBP4 of liver and adipose tissues in adolescent obese rats].

OBJECTIVE

To exploer effects of conjugated linoleic acid and exercise on RBP4 of liver and adipose tissues in adolescent obese rats.

METHODS

8 adolescent SD rats were selected as blank control (C) with normal diet. Obese rats were modeled with high fat feeding for 7 weeks. 32 obese rats were selected and randomly divided into 4 groups: control group (OC), CLA group ( OCC), exercise group (OM), exercise combined with CLA group (OMC). Exercise treatment were performed on animal treadmill with running speed of 21 - 25 m/min, 60 min/every time. For CLA treatment, each rat was fed 1.6 g/kg, one time/day, 5 times/week, for 8 weeks. Animals were anesthesia treated after experiment, blood and body tissues were collected. The content of blood glucose was measured, the insulin sensitivity was calculated, testing the expression of RBP4 mRNA in visceral adipose tissue, expression of RBP4 protein in liver cells and plasma RBP4 concentration.

RESULT

(1) Weight, weight growth rate and body fat percentage of OM, OMC groups were lower than OC, OCC groups (P < 0.01). (2) Blood glucose content of OM, OMC groups were significantly lower than OC, OCC groups (P < 0.01), while insulin sensitivity of OM, OMC groups was higher than OC, OCC groups (P < 0.01). (3) Expression of RBP4 mRNA of OM, OMC groups in visceral adipose tissue was lower than OC, OCC groups (P < 0.01). Expression of RBP4 protein of OM, OMC groups in liver cells was significantly lower than OC, OCC groups. RBP4 concentration in plasma of OM, OMC groups was lower than OC, OCC groups (P < 0.01). (4) Weight, weight growth rate, insulin sensitivity of OMC group were higher than OM group, but the percentage of body fat, blood glucose content and the expression of RBP4 were lower than OM group, there were no significant differences between them.

CONCLUSION

Simple exercise and exercise combined with CLA conld significantly reduced adolescent obese rats weight, weight growth rate, percentage of body fat, blood glucose content and also reduced RBP4 mRNA expression in visceral adipose tissue, RBP4 protein expression in liver tissue, RBP4 level in plasma,but can increase sensitivity to insulin, in a word, the treatment of exercise and exercise combine with CLA is better than the simple complement of CLA to adolescent obese rats.