Cui Jiaqin, Bai Youping, Wu Lin, Sun Mingming, Lin Chan, Zhang Haoyue, Su Min, Song Weiwei
College of Physical Education, Anhui Normal University, Wuhu 241003, China.
Wei Sheng Yan Jiu. 2017 Jul;46(4):602-609.
To explore the effects of exercise and resveratrol on retinol binding protein 4( RBP4) mRNA and protein expression in visceral adipose tissue, plasma RBP4 concentration and blood glucose and insulin sensitivity of aged obese rats.
To establish elderly obese SD rat model, 6 aged rats of natural growth were selected normal group, 24 obese rats were randomly divided into four groups: obesity control group, exercise group, resveratrol group, exercise combined with resveratrol group. For Resveratrol treatment, each rat was fed 52. 5 mg/( kg d), 5 times/week, continuous intervention for 8 weeks. After intervention, measured blood glucose, insulin sensitivity, visceral adipose tissue RBP4 mRNA expression( qRT-PCR), protein expression( Western blot) and plasma RBP4 concentration( ELISA).
After 8week intervention, ( 1) RBP4 mRNA expression: The obesity group( 2. 63 ± 0. 45) was higher than that of the normal group( 2. 10 ± 0. 15)( P < 0. 05). The resveratrol group( 1. 84 ± 0. 33), exercise group( 1. 91 ± 0. 15), and exercise combined with resveratrol group( 2. 131 ± 0. 111) were lower than that of the obesity group( P < 0. 05, P < 0. 01). ( 2) RBP4 protein expression: The obesity group( 1. 346 ± 0. 025) was higher than that of the normal group( 1. 196 ± 0. 017)( P < 0. 01). The exercise group( 1. 025 ± 0. 006)was lower than that of the obesity group( P < 0. 01), The resveratrol group( 0. 735 ±0. 015) and exercise combined with resveratrol group( 0. 701 ± 0. 018) were lower than that of the exercise group( P < 0. 01). The exercise combined with resveratrol group was lower than that of resveratrol group( P < 0. 05). ( 3) Plasma RBP4 concentration: The obesity group [( 16. 00 ± 1. 54) μg/L]was higher than that of the normal group [( 13. 02± 2. 20) μg/L]( P < 0. 01). The exercise group [( 14. 76 ± 1. 56) μg/L] was lower than that of the obesity group, but the difference was not statistically significant. The resveratrol group [( 13. 59 ± 0. 07) μg/L]and exercise combined with resveratrol group[( 12. 98 ± 1. 69) μg/L] were lower than that of the obesity group( P < 0. 05, P <0. 01). ( 4) Blood glucose: The obesity group [( 17. 93 ± 6. 09) mmol/L]was higher than that of the normal group [( 11. 64 ± 3. 57) mmol/L ]( P < 0. 01). The exercise group [( 13. 36 ± 1. 82) mmol/L]was lower than that of the obesity group( P < 0. 05), the resveratrol group [( 15. 24 ± 2. 19) mmol/L] and exercise combined with resveratrol group [( 13. 95 ± 2. 26) mmol/L] were lower than that of the obesity group, but the difference was not statistically significant( P > 0. 05). ( 5) Insulin sensitivity: The obesity group( 0. 37 ± 0. 02) was lower than that of the normal group( 0. 39 ± 0. 02)( P < 0. 05). The resveratrol group( 0. 38 ± 0. 01) and the exercise group( 0. 39 ± 0. 02)were higher than that of the obesity group, but the difference was not statistically significant, the exercise combined with resveratrol group( 0. 39 ± 0. 15) was higher than that of the obesity group( P < 0. 05). Above indexes( except protein expression) were no significant difference between the each intervene groups( P > 0. 05).
RBP4 mRNA expression and protein expression in the visceral adipose tissue of aged obese rats, plasma RBP4 concentration and blood glucose were increased, and insulin sensitivity was decreased. Exercise and resveratrol could reduce the expression of RBP4 mRNA and protein expression and plasma RBP4 concentration. The simple exercise could significantly reduce the blood glucose and exercise combined with resveratrol could significantlyimprove insulin sensitivity. The decrease in protein expression, plasma RBP4 concentration and improve insulin sensitivity, exercise combined with resveratrol showed synergy.
探讨运动和白藜芦醇对老年肥胖大鼠内脏脂肪组织视黄醇结合蛋白4（RBP4）mRNA和蛋白表达、血浆RBP4浓度以及血糖和胰岛素敏感性的影响。
选取6只自然生长的老年大鼠作为正常组，建立老年肥胖SD大鼠模型，将24只肥胖大鼠随机分为四组：肥胖对照组、运动组、白藜芦醇组、运动联合白藜芦醇组。白藜芦醇治疗时，每只大鼠按52.5mg/（kg·d）给药，每周5次，连续干预8周。干预后，检测血糖、胰岛素敏感性、内脏脂肪组织RBP4 mRNA表达（qRT-PCR）、蛋白表达（Western blot）及血浆RBP4浓度（ELISA）。
干预8周后，（1）RBP4 mRNA表达：肥胖组（2.63±0.45）高于正常组（2.10±0.15）（P<0.05）。白藜芦醇组（1.84±0.33）、运动组（1.91±0.15）、运动联合白藜芦醇组（2.131±0.111）低于肥胖组（P<0.05，P<0.01）。（2）RBP4蛋白表达：肥胖组（1.346±0.025）高于正常组（1.196±0.017）（P<0.01）。运动组（1.025±0.006）低于肥胖组（P<0.01），白藜芦醇组（0.735±0.015）、运动联合白藜芦醇组（0.701±±0.018）低于运动组（P<0.01）。运动联合白藜芦醇组低于白藜芦醇组（P<0.05）。（3）血浆RBP4浓度：肥胖组[（16.00±1.54）μg/L]高于正常组[（13.02±2.20）μg/L]（P<0.01）。运动组[（14.76±1.56）μg/L]低于肥胖组，但差异无统计学意义。白藜芦醇组[（13.59±0.07）μg/L]、运动联合白藜芦醇组[（12.98±1.69）μg/L]低于肥胖组（P<0.05，P<0.01）。（4）血糖：肥胖组[（17.93±6.09）mmol/L]高于正常组[（11.64±3.57）mmol/L]（P<0.01）。运动组[（13.36±1.82）mmol/L]低于肥胖组（P<0.05），白藜芦醇组[（15.24±2.19）mmol/L]、运动联合白藜芦醇组[（13.95±2.26）mmol/L]低于肥胖组，但差异无统计学意义（P>0.05）。（5）胰岛素敏感性：肥胖组（0.37±0.02）低于正常组（0.39±0.02）（P<0.05）。白藜芦醇组（0.38±0.01）、运动组（0.39±0.（02）高于肥胖组，但差异无统计学意义，运动联合白藜芦醇组（0.39±0.15）高于肥胖组（P<0.05）。各干预组上述指标（蛋白表达除外）差异无统计学意义（P>0.05）。
老年肥胖大鼠内脏脂肪组织RBP4 mRNA表达和蛋白表达、血浆RBP4浓度及血糖升高，胰岛素敏感性降低。运动和白藜芦醇可降低RBP4 mRNA表达、蛋白表达及血浆RBP4浓度。单纯运动可显著降低血糖，运动联合白藜芦醇可显著提高胰岛素敏感性。在降低蛋白表达、血浆RBP4浓度及提高胰岛素敏感性方面，运动联合白藜芦醇表现出协同作用。
