不同强度运动对青春期肥胖大鼠血糖、胰岛素敏感性及视黄醇结合蛋白4的影响

[Effects of different intensity exercise on blood glucose, adolescent obesity rats insulin sensitivity and RBP4].

作者信息

Cui Jianfei, Bai Youping, Li Meng, Xu Xiaodong, Dai Yuanyuan, Zhang Jing

出版信息

Wei Sheng Yan Jiu. 2014 Jul;43(4):535-40.

PMID:25199277

Abstract

OBJECTIVE

To discuss the effects of different intensity exercise on blood glucose, adolescent obesity rats insulin sensitivity and RBP4.

METHODS

The model of rats after 7 weeks, 32 rabbits of obesity prone rats were randomly divided into 4 groups, 8 rats in each group, respectively, control group, low intensity exercise group, moderate intensity exercise group, high intensity exercise group. High strength low in animal treadmill running speed respectively for 15 - 18, 21 - 25 and 28 - 32 m/min, 5 times/week, 60 min/times, for 8 consecutive weeks. Anesthesia for blood and tissue collection after exercise intervention, blood glucose, blood insulin, blood RBP4 concentration, liver RBP4 protein expression and visceral adipose tissue RBP4 mRNA expression.

RESULT

The 8 week exercise intervention, low, high strength, in the sugar group was significantly lower than the control group (P < 0.01). Insulin sensitivity was higher than that of the control group (P < 0.01), insulin was lower than the control group difference. Expression of visceral adipose tissue RBP4 mRNA was low, medium and high strength group was lower than the control group, the expression was significantly down regulated (P < 0.01), in the high intensity group, low intensity group lowered significantly (P < 0.01). RBP4 higher than exercise group as control group in the expression of liver tissue protein, in the high intensity group, less the amount of expression. Plasma RBP4 concentration of high strength group was significantly lower than that of the control group (P < 0.01), high strength, low intensity group were lower than those in group (P < 0.01).

CONCLUSION

Through the different intensity exercise intervention can reduce blood glucose in obese rats, the plasma RBP4 concentration, improve insulin sensitivity, lower expression of RBP4 mRNA in adipose tissues, liver protein expression decreased, with the exercise intensity increased significantly.

摘要

目的

探讨不同强度运动对血糖、青春期肥胖大鼠胰岛素敏感性及视黄醇结合蛋白4（RBP4）的影响。

方法

7周龄大鼠造模后，将32只肥胖倾向大鼠随机分为4组，每组8只，分别为对照组、低强度运动组、中等强度运动组、高强度运动组。在动物跑步机上分别以低、中、高强度的速度15 - 18、21 - 25和28 - 32米/分钟跑步，每周5次，每次60分钟，连续8周。运动干预后麻醉取血及组织，检测血糖、血胰岛素、血RBP4浓度、肝脏RBP4蛋白表达及内脏脂肪组织RBP4 mRNA表达。

结果

8周运动干预后，低、高强度组血糖显著低于对照组（P < 0.01）。胰岛素敏感性高于对照组（P < 0.01），胰岛素低于对照组差异有统计学意义。内脏脂肪组织RBP4 mRNA表达低、中、高强度组均低于对照组，表达显著下调（P < 0.01），高强度组较中强度组、低强度组下调显著（P < 0.01）。运动组肝脏组织蛋白表达RBP4高于对照组，高强度组表达量较少。高强度组血浆RBP4浓度显著低于对照组（P < 0.01），高强度组低于低强度组（P < 0.01）。