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[2011年中国微生物实验室需氧平板计数质量控制结果分析]

[Analysis on 2011 quality control results on aerobic plate count of microbiology laboratories in China].

作者信息

Han Haihong, Li Ning, Li Yepeng, Fu Ping, Yu Dongmin, Du Chunming, Guo Yunchang

出版信息

Wei Sheng Yan Jiu. 2015 Jan;44(1):73-6.

Abstract

OBJECTIVE

To test the aerobic plate count examining capability of microbiology laboratories, to ensure the accuracy and comparability of quantitative bacteria examination results, and to improve the quality of monitoring.

METHODS

The 4 different concentration aerobic plate count piece samples were prepared and noted as I, II, III and IV. After homogeneity and stability tests, the samples were delivered to monitoring institutions. The results of I, II, III samples were logarithmic transformed, and evaluated with Z-score method using the robust average and standard deviation. The results of IV samples were evaluated as "satisfactory" when reported as < 10 CFU/piece or as "not satisfactory" otherwise. Pearson χ2 test was used to analyze the ratio results.

RESULTS

309 monitoring institutions, which was 99.04% of the total number, reported their results. 271 institutions reported a satisfactory result, and the satisfactory rate was 87.70%. There was no statistical difference in satisfactory rates of I, II and III samples which were 81.52%, 88.30% and 91.40% respectively. The satisfactory rate of IV samples was 93.33%. There was no statistical difference in satisfactory rates between provincial and municipal CDC.

CONCLUSION

The quality control program has provided scientific data that the aerobic plate count capability of the laboratories meets the requirements of monitoring tasks.

摘要

目的

检测微生物实验室的需氧平板计数检测能力,确保定量细菌检测结果的准确性和可比性,提高监测质量。

方法

制备4种不同浓度的需氧平板计数片样本,分别标记为I、II、III和IV。经过均匀性和稳定性测试后,将样本分发给监测机构。对I、II、III样本的结果进行对数转换,采用稳健均值和标准差的Z评分法进行评估。IV样本结果报告为<10 CFU/片时判定为“满意”,否则判定为“不满意”。采用Pearson χ2检验分析比率结果。

结果

309家监测机构上报了结果,占总数的99.04%。271家机构报告结果满意,满意率为87.70%。I、II、III样本的满意率分别为81.52%、88.30%和91.40%,差异无统计学意义。IV样本的满意率为93.33%。省级和市级疾控中心的满意率差异无统计学意义。

结论

质量控制程序提供了科学数据,表明实验室的需氧平板计数能力符合监测任务要求。

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