Han Haihong, Li Fengqin, Xu Jin, Bai Li, Wang Wei, Hu Yujie, Li Yepeng, Yu Dongmin
Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Centre for Food Safety Risk Assessment, Beijing 100021, China.
Wei Sheng Yan Jiu. 2017 May;46(3):429-433.
To get a baseline of 32 provincial center for disease control and prevention( CDC) microbiology laboratory in the aspect of quantitative test ability of Staphylococcus aureu, qualitative test ability of diarrheagenic Escherichia coli and unknown intestinal pathogen, and to comprehend the quality of microbiology testing.
Two different concentration samples of Staphylococcus aureus I and II were made. Diarrheagenic Escherichia coli and unknown intestinal pathogen samples were transported using the special semi-solid AGAR transport medium, in the form of four pure different serotypes each. The results of Staphylococcus aureu were logarithmic transformed, and evaluated with Z-score method using the average and standard deviation. The reporting results of diarrheagenic Escherichia coli and unknown intestinal pathogen were evaluated as right or wrong, and non-reporting were evaluated as missing.
The satisfaction rate of Staphylococcus aureus was 82. 81%. The accuracy rate of diarrheagenic Escherichia coli was 45. 16%. Of the 32 results of unknown intestinal pathogen, 29 CDCs reported correctSalmonella serotypes, and the accuracy rate was 90. 63%. Combined the three quality control results, 9 CDCs reached the accurate results, with the accuracy rate of 28. 13%.
32 provincial CDCs have the identification ability of Salmonella serotyping and qualitative test ability of Staphylococcus aureu, while they need to strength diarrheagenic Escherichia coli identification and serotyping ability.
了解32家省级疾病预防控制中心（CDC）微生物实验室在金黄色葡萄球菌定量检测能力、致泻性大肠埃希菌及肠道未知病原菌定性检测能力方面的基线情况，掌握微生物检测质量。
制备两种不同浓度的金黄色葡萄球菌Ⅰ和Ⅱ样本。致泻性大肠埃希菌和肠道未知病原菌样本采用特殊的半固体琼脂运输培养基运输，每种均为4种不同的纯血清型。金黄色葡萄球菌的检测结果进行对数转换，采用Z分数法以均值和标准差进行评价。致泻性大肠埃希菌和肠道未知病原菌的报告结果按正确或错误进行评价，未报告的按缺失进行评价。
金黄色葡萄球菌的满意率为82.81%。致泻性大肠埃希菌的准确率为45.16%。在32份肠道未知病原菌检测结果中，29家CDC报告了正确的沙门菌血清型，准确率为90.63%。综合三项质量控制结果，9家CDC达到准确结果，准确率为28.13%。
32家省级CDC具备沙门菌血清分型鉴定能力和金黄色葡萄球菌定性检测能力，但致泻性大肠埃希菌的鉴定和血清分型能力有待加强。
