Cherian Aleena Mary, Snima K S, Kamath C Ravindranath, Nair Shantikumar V, Lakshmanan Vinoth-Kumar
Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi Campus, Kerala 682041, India.
Holistic Medicine Department, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi Campus, Kerala 682041, India.
Biomed Pharmacother. 2015 Apr;71:201-9. doi: 10.1016/j.biopha.2015.02.016. Epub 2015 Mar 5.
Prostate cancer has been diagnosed as the second most frequent and the sixth among the cancer causing deaths among men worldwide. There is a limited scope for the prevalent therapies as prostate cancer advances and they present adverse aftermaths that have put way for us to delve into naturally available anticancer agents. The main objective of the present work is to compile the advantages of ayurvedic herbal formulations with modern technology. Baliospermum montanum is a plant that is used in ayurveda for the treatment of cancer and the plant is studied to possess various constituents in it that are responsible for its anticancer activity. Stable nanoparticles of B. montanum were prepared from both the aqueous and ethanolic extracts of the plant and its cytotoxic effects were studied on prostate cancer and normal cell lines. Size analysis by DLS and SEM revealed the average size of nanoparticles prepared was 100±50 nm and 150±50 nm for the nanoparticles prepared from aqueous and ethanolic extract respectively. In vitro cytotoxicity showed a concentration and time dependent toxicity on prostate cancer cells with cell viability of 22% and 6% with maximum concentration of aqueous and ethanolic nanoparticles respectively, in 48 h. In vitro hemolysis assay confirmed that the prepared nanoparticles were compatible with blood with no occurrence of hemolysis. The nanoparticles showed a significant reduction in the colony forming ability and wound healing capacity of the prostate cancer cells. These studies hold the anti cancer potential of the B. montanum nanoparticles making it an important candidate for prostate cancer therapy.
前列腺癌已被诊断为全球男性中第二常见的癌症，在癌症致死原因中位列第六。随着前列腺癌病情发展，现有治疗方法的适用范围有限，且会产生不良后果，这促使我们深入研究天然抗癌药物。本研究的主要目的是将阿育吠陀草药配方与现代技术的优势相结合。麻疯树是一种在阿育吠陀医学中用于治疗癌症的植物，经研究发现该植物含有多种具有抗癌活性的成分。从该植物的水提取物和乙醇提取物中制备了麻疯树稳定纳米颗粒，并研究了其对前列腺癌细胞系和正常细胞系的细胞毒性作用。通过动态光散射（DLS）和扫描电子显微镜（SEM）进行的尺寸分析表明，从水提取物和乙醇提取物制备的纳米颗粒的平均尺寸分别为100±50纳米和150±50纳米。体外细胞毒性实验表明，纳米颗粒对前列腺癌细胞具有浓度和时间依赖性毒性，在48小时内，水相和乙醇相纳米颗粒的最大浓度分别使细胞活力降至22%和6%。体外溶血试验证实，所制备的纳米颗粒与血液相容，未发生溶血现象。纳米颗粒显著降低了前列腺癌细胞的集落形成能力和伤口愈合能力。这些研究表明麻疯树纳米颗粒具有抗癌潜力，使其成为前列腺癌治疗的重要候选药物。
