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用生长抑制剂区分溶血巴斯德氏菌生物型A和T

Differentiation of Pasteurella haemolytica biotypes A and T with growth inhibitors.

作者信息

Olmos A, Biberstein E L

出版信息

J Clin Microbiol. 1979 Aug;10(2):231-4. doi: 10.1128/jcm.10.2.231-234.1979.

Abstract

Differentiation of type A and type T cultures of Pasteurella haemolytica was accomplished by paper disks containing 0.3 microgram of penicillin G, which produced zones of inhibition larger than 10 mm with type A but not with type T strains. Basic fuchsin (0.2 microgram/ml), brilliant green (0.005 microgram/ml), methylene blue (3.1 micrograms/ml) in brain heart infusion broth permitted the growth of type T but that of type A isolates.

摘要

溶血巴斯德菌A 型和T 型培养物的鉴别是通过含有0.3微克青霉素G 的纸片来完成的,青霉素G 对A 型菌株产生的抑菌圈大于10毫米,而对T 型菌株则不产生抑菌圈。在脑心浸液肉汤中加入碱性品红(0.2微克/毫升)、亮绿(0.005微克/毫升)、亚甲蓝(3.1微克/毫升),可使T 型菌株生长,而A 型菌株则不能生长。

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