Slížová Magdaléna, Nemcová Radomíra, Mad'ar Marián, Hadryová Jana, Gancarčíková Soňa, Popper Miroslav, Pistl Juraj
Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovak Republic.
Can J Microbiol. 2015 Jun;61(6):437-46. doi: 10.1139/cjm-2015-0007. Epub 2015 Apr 16.
In this study, the biofilm-forming potential of intestinal Lactobacillus reuteri strains under different culture conditions was characterized by microtiter plate biofilm assays. Moreover, the spatial organization of exogenously applied L. reuteri L2/6 (a pig isolate) at specific locations in gastrointestinal tract of monoassociated mice was investigated by fluorescence in situ hybridization. We did not detect biofilm formation by tested strains in nutrient-rich de Man-Rogosa-Sharpe (MRS) medium. On the contrary, a highly positive biofilm formation was observed in medium with lower accessibility to the carbon sources and lack of salts. The results obtained confirmed the significant role of Tween 80 and the quantity and nature of the sugars in the growth medium in biofilm formation. The omission of Tween 80 in MRS medium favored the formation of biofilm. Abundant biofilm formation was detected in the presence of lactose, galactose, and glucose. However, a gradual increase in sugar concentration triggered a significant decrease in biofilm formation. In addition, conditions related to the gastrointestinal environment, such as low pH and the presence of bile and mucins, highly modulated biofilm production. This effect seems to be dependent on the specificity and properties of the medium used for cultivation. From the evidence provided by this study we conclude that the biofilm formation capacity of L. reuteri is strongly dependent on the environmental factors and culture medium used.
在本研究中，通过微量滴定板生物膜试验对不同培养条件下肠道罗伊氏乳杆菌菌株形成生物膜的潜力进行了表征。此外，通过荧光原位杂交研究了外源性应用的罗伊氏乳杆菌L2/6（一种猪分离株）在单联小鼠胃肠道特定位置的空间组织。我们在营养丰富的德氏乳酸杆菌-罗格斯-夏普（MRS）培养基中未检测到受试菌株形成生物膜。相反，在碳源可及性较低且缺乏盐分的培养基中观察到高度阳性的生物膜形成。所获得的结果证实了吐温80以及生长培养基中糖的数量和性质在生物膜形成中的重要作用。MRS培养基中省略吐温80有利于生物膜的形成。在乳糖、半乳糖和葡萄糖存在的情况下检测到大量生物膜形成。然而，糖浓度的逐渐增加引发生物膜形成的显著减少。此外，与胃肠道环境相关的条件，如低pH值以及胆汁和粘蛋白的存在，对生物膜产生有高度调节作用。这种效应似乎取决于用于培养的培养基的特异性和性质。根据本研究提供的证据，我们得出结论，罗伊氏乳杆菌的生物膜形成能力强烈依赖于环境因素和所用的培养基。
