Du Li, Li Yongpeng, Yao Yao, Zhang Liwei
School of Life Science and Technology, Nanyang Normal University, Nanyang City, Henan Province, P. R. China.
PLoS One. 2015 May 11;10(5):e0127215. doi: 10.1371/journal.pone.0127215. eCollection 2015.
A simple and efficient plantlet regeneration protocol via direct organogenesis was established for camphor tree (Cinnamomum camphora L.). Stem segments with one node (SN explants) from embryo-cultured seedlings (EC seedlings) were used as explants. Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2, 4-dichlorophenoxyacetic acid and 2.0 mg/L 6-benzyladenine was used to induce cotyledonary embryo germination. This medium was also used for EC seedlings propagation and adventitious bud induction from SN explants. Regenerated plantlets were cultured on hormone-free MS medium for elongation and root induction. The regeneration capability of SN explants was compared by using EC seedling lines established in this research. EC seedling line EL6 exhibited the highest adventitious bud induction frequency (91.7%) and the highest number of buds per responding explant (5.2), which was considered as the most efficient EC seedling line for further gene transformation research.
通过直接器官发生建立了一种简单高效的樟树（Cinnamomum camphora L.）植株再生体系。以胚培养苗（EC苗）的单节茎段（SN外植体）作为外植体。添加0.5 mg/L 2,4-二氯苯氧乙酸和2.0 mg/L 6-苄基腺嘌呤的Murashige和Skoog（MS）培养基用于诱导子叶胚萌发。该培养基也用于EC苗的增殖以及SN外植体不定芽的诱导。再生植株在无激素的MS培养基上培养以进行伸长和生根诱导。通过使用本研究中建立的EC苗系比较SN外植体的再生能力。EC苗系EL6表现出最高的不定芽诱导频率（91.7%）和每个响应外植体的最高芽数（5.2），被认为是用于进一步基因转化研究的最有效的EC苗系。
