Color changes produced by histological alterations in burned teeth can provide conclusive forensic information on the temperature of exposure. The objective was to correlate heat-induced color changes in incinerated teeth with increases in temperature (to 1200°C). Spectrophotometry was used to measure lightness, chromaticity (a* and b*), whiteness, and yellowness in 80 teeth heated at temperatures of 100, 200, 400, 600, 800, 1000, or 1200°C for 60 min. Chromaticity a* was reduced at 100°C and lightness at 200 and 400°C, while chromaticity b* and yellowness were reduced at 400 and 600°C. Higher temperatures (800, 1000, and 1200°C) produced progressive increases in lightness and whiteness but reductions in chromaticity b* and yellowness. The accuracy of color values to determine the temperature of exposure was determined by Receiver Operating Characteristic analysis. High accuracy was shown by lightness, chromaticity b* and yellowness values for temperatures between 800° and 1200°C, by whiteness for temperatures of 1000° and 1200°C, and by lightness for temperatures of 200° and 400°C, with sensitivity and specificity values ranging from 90% to 100%. According to these results, colorimetric analysis of incinerated teeth can be used to estimate the temperature of exposure with high accuracy, with lightness being the most useful variable.