Rubio Leticia, Díaz-Vico Ramona, Smith-Fernández Inés, Smith-Fernández Aníbal, Suárez Juan, Martin-de-Las-Heras Stella, Santos Ignacio
Department of Forensic and Legal Medicine, Instituto de Investigación Biomédica de Málaga-IBIMA (CE-18), University of Malaga, 29071 Malaga, Spain.
Department of Human Anatomy, University of Malaga, 29071 Malaga, Spain.
Diagnostics (Basel). 2020 Nov 20;10(11):979. doi: 10.3390/diagnostics10110979.
Heated-bone color changes may provide information about temperature of exposure, with interest for anthropologists and forensic experts. The aim of this study was to assess heat-induced color changes by spectrophotometry in cortical and medullar human bones heated at different temperatures and times. CIELAB (International Commission on Illumination-LAB) color parameters (L*, a*, and b*) and whiteness (WI) and yellowness (YI) indexes were obtained by spectrophotometry in the cortical and medullar zones of 36 bone sections exposed at 200, 400, 600, and 800 °C for 30 and 60 min. The accuracy of color-based temperature estimations was evaluated by Receiver Operating Characteristics (ROC) analysis. Chromaticity a* showed the best significant discrimination power with the area under the ROC curve (AUC) values ranged from 0.9 to 1.0 in cortical zones and 0.7 to 1.0 in medullar zones for all temperatures of exposures and both time of exposures. Chromaticity b*, and WI and YI indexes showed an AUC of 1.0 at 400, 600, and 800 °C for 30 and 60 min in the cortical and medullar zones. The spectrophotometric color parameters provided a highly accurate estimation of the temperature of exposure to discriminate between temperatures and exposure times in the cortical and medullar zones. Spectrophotometric bone color measurement in cortical and medullar zones can be an objective and reproducible method to estimate the temperature of exposition, and it can be considered useful for forensic and anthropological purposes.
加热骨骼的颜色变化可能会提供有关暴露温度的信息，这引起了人类学家和法医专家的兴趣。本研究的目的是通过分光光度法评估在不同温度和时间下加热的人类皮质骨和髓质骨中热诱导的颜色变化。通过分光光度法在36个骨切片的皮质和髓质区域中获取CIELAB（国际照明委员会-LAB）颜色参数（L*、a和b）以及白度（WI）和黄度（YI）指数，这些骨切片分别在200、400、600和800°C下暴露30分钟和60分钟。通过受试者工作特征（ROC）分析评估基于颜色的温度估计的准确性。色度a显示出最佳的显著判别能力，在所有暴露温度和暴露时间下，皮质区域的ROC曲线下面积（AUC）值范围为0.9至1.0，髓质区域为0.7至1.0。色度b以及WI和YI指数在皮质和髓质区域中，在400、600和800°C下暴露30分钟和60分钟时的AUC为1.0。分光光度颜色参数能够高度准确地估计暴露温度，以区分皮质和髓质区域中的温度和暴露时间。皮质和髓质区域的分光光度骨颜色测量可以是一种客观且可重复的方法来估计暴露温度，并且可以认为对法医和人类学目的有用。
