Ramadan Gamal, El-Beih Nadia M, Talaat Roba M, Abd El-Ghffar Eman A
Zoology Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat City University, Sadat City, Egypt.
Int J Rheum Dis. 2017 Feb;20(2):203-213. doi: 10.1111/1756-185X.12666. Epub 2015 May 12.
Recently, there has been an increasing interest in tea (Camellia sinensis) as a protective agent against inflammatory diseases. Here, we evaluated/compared the anti-inflammatory activity of two different doses (0.5 and 1.0 g/kg body weight) of green tea aqueous extract (GTE, rich in catechins) and black tea aqueous extract (BTE, rich in theaflavins and thearubigins) in rat adjuvant-induced arthritis (AIA).
Adjuvant-induced arthritis rat model received orally/daily distilled water as vehicle, indomethacin (1.0 mg/kg body weight; a non-steroidal/anti-inflammatory drug), or tea aqueous extracts (for 28 or 14 consecutive days starting from day 0 or 14 of arthritis induction, respectively).
The present study showed that only the high dose of GTE (from day 0) significantly alleviated (P < 0.05-0.001) all complications shown in arthritic rats, including synovial joint inflammation, elevation in erythrocyte sedimentation rate, blood leukocytosis (due to lymphocytosis and neutrocytosis), and changes in weight/cellularity of lymphoid organs. The anti-arthritic activity of the high dose of GTE (from day 0) was comparable (P > 0.05) with that of indomethacin (12.9-53.8 vs. 9.5-48.4%, respectively) and mediated by significantly decreasing and down-regulating (P < 0.001) the systemic production of pro-inflammatory cytokines and the expression of chemokine receptor-5 in synovial tissues, respectively. Moreover, the anti-arthritic activity of tea aqueous extracts was in the following order: high dose of GTE > low dose of GTE ≥ high dose of BTE > low dose of BTE.
The present study proved the anti-inflammatory activity of GTE over BTE and equal to that of indomethacin in AIA rat model.
最近，茶（茶树）作为一种抗炎症疾病的保护剂受到越来越多的关注。在此，我们评估/比较了两种不同剂量（0.5和1.0克/千克体重）的绿茶水提取物（GTE，富含儿茶素）和红茶水提取物（BTE，富含茶黄素和茶红素）对大鼠佐剂性关节炎（AIA）的抗炎活性。
佐剂性关节炎大鼠模型每天口服蒸馏水作为赋形剂、吲哚美辛（1.0毫克/千克体重；一种非甾体抗炎药）或茶水提取物（分别从关节炎诱导的第0天或第14天开始连续28天或14天）。
本研究表明，只有高剂量的GTE（从第0天开始）能显著减轻（P<0.05 - 0.001）关节炎大鼠出现的所有并发症，包括滑膜关节炎症、红细胞沉降率升高、血液白细胞增多（由于淋巴细胞增多和中性粒细胞增多）以及淋巴器官重量/细胞数量的变化。高剂量的GTE（从第0天开始）的抗关节炎活性与吲哚美辛相当（P>0.05）（分别为12.9 - 53.8%对9.5 - 48.4%），并且分别通过显著降低和下调（P<0.001）促炎细胞因子的全身产生以及滑膜组织中趋化因子受体 - 5的表达来介导。此外，茶水提取物的抗关节炎活性顺序如下：高剂量的GTE>低剂量的GTE≥高剂量的BTE>低剂量的BTE。
本研究证明了在AIA大鼠模型中，GTE的抗炎活性优于BTE且与吲哚美辛相当。
