Park Sooyeon, Ha Min-Ji, Yoon Sun Young, Jung Yong-Taek, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Jan;67(1):25-30. doi: 10.1099/ijsem.0.001562. Epub 2017 Feb 6.
A Gram-stain-negative, non-motile, aerobic and coccoid, ovoid or rod-shaped bacterium, designated GHTF-24T, was isolated from a tidal flat on the South Sea, South Korea, and subjected to polyphasic taxonomic study. The novel strain grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain GHTF-24T fell within the clade comprising the type strains of species of the genus Roseovarius. Strain GHTF-24T exhibited 16S rRNA gene sequence similarity values of 97.0 % to each of Roseovariushalotolerans HJ50T and Roseovariuspacificus 81-2T and of 91.6-96.5 % to the type strains of the other species of the genus Roseovarius. Strain GHTF-24T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids of strain GHTF-24T were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain GHTF-24T was 62.3 mol%, and its mean DNA-DNA relatedness values with the type strains of R. halotolerans and R.pacificus were 13-16 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain GHTF-24T is separated from other recognized species of the genus Roseovarius. On the basis of the data presented, strain GHTF-24T is considered to represent a novel species of the genus Roseovarius, for which the name Roseovarius aestuariivivens sp. nov. is proposed. The type strain is GHTF-24T (=KCTC 52454T=NBRC 112420T).
从韩国南海的潮滩中分离出一株革兰氏染色阴性、不运动、需氧的球菌、卵形菌或杆菌，命名为GHTF-24T，并对其进行了多相分类研究。该新菌株在30℃、pH 7.0-8.0以及2.0-3.0%（w/v）NaCl条件下生长最佳。在基于16S rRNA基因序列构建的邻接系统发育树中，菌株GHTF-24T位于包含玫瑰色杆菌属各物种模式菌株的进化分支内。菌株GHTF-24T与嗜盐玫瑰色杆菌HJ50T和太平洋玫瑰色杆菌81-2T的16S rRNA基因序列相似性值均为97.0%，与玫瑰色杆菌属其他物种的模式菌株的相似性值为91.6-96.5%。菌株GHTF-24T以Q-10作为主要泛醌，以C18 : 1ω7c作为主要脂肪酸。菌株GHTF-24T的主要极性脂类为磷脂酰胆碱、磷脂酰甘油、双磷脂酰甘油、一种未鉴定的氨基脂类和一种未鉴定的脂类。菌株GHTF-24T的DNA G+C含量为62.3 mol%，其与嗜盐玫瑰色杆菌和太平洋玫瑰色杆菌模式菌株的平均DNA-DNA相关性值为13-16%。其不同的表型特征，以及系统发育和遗传独特性，表明菌株GHTF-24T与玫瑰色杆菌属其他已确认的物种不同。根据所提供的数据，菌株GHTF-24T被认为代表玫瑰色杆菌属的一个新物种，为此提出新名称嗜河口玫瑰色杆菌（Roseovarius aestuariivivens sp. nov.）。模式菌株为GHTF-24T（=KCTC 52454T=NBRC 112420T）。
