Cheung A K
Medical Service, Veterans Affairs Medical Center, Salt Lake City, UT 84148.
Am J Kidney Dis. 1989 Dec;14(6):472-7. doi: 10.1016/s0272-6386(89)80147-7.
Hemodialysis using polyacrylonitrile (PAN) membranes has been reported to be associated with depletion of complement in the plasma, yet the increase in plasma C3a antigen concentrations and the degree of leukopenia are modest. These observations suggest that PAN membranes may have a large propensity to adsorb native complement proteins; as a consequence, complement depletion can occur without activation. In the present study, we observed that incubation of human serum in the presence of PAN membrane resulted in a 50% loss of serum hemolytic activity of C3. When radiolabeled purified components were offered, PAN membranes were found to adsorb C3 and C5 in a dose-dependent manner. Adsorption of these proteins by PAN was more than 20 times greater than adsorption by cuprophan, cellulose acetate or Hemophan (Akzo, formerly Enka, Wuppertal, FRG) at all concentrations examined. These results suggest that depletion of complement when serum is exposed to hemodialysis membranes may result from adsorption of complement components onto the membrane surfaces and does not necessarily indicate complement activation.
据报道，使用聚丙烯腈（PAN）膜进行血液透析与血浆中补体的消耗有关，但血浆C3a抗原浓度的增加和白细胞减少的程度较小。这些观察结果表明，PAN膜可能有很大的倾向吸附天然补体蛋白；因此，补体消耗可能在未激活的情况下发生。在本研究中，我们观察到在PAN膜存在的情况下孵育人血清会导致C3的血清溶血活性损失50%。当提供放射性标记的纯化成分时，发现PAN膜以剂量依赖的方式吸附C3和C5。在所有检测浓度下，PAN对这些蛋白质的吸附比铜仿膜、醋酸纤维素膜或Hemophan膜（阿克苏公司，原恩卡公司，德国伍珀塔尔）大20倍以上。这些结果表明，当血清暴露于血液透析膜时，补体的消耗可能是由于补体成分吸附到膜表面，并不一定表明补体被激活。
