FibEnTech - Fiber Materials and Environmental Technologies - Research Unit, Faculty of Engineering, University of Beira Interior, 6201-001 Covilhã, Portugal.
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal; Health Sciences Research Centre, University of Beira Interior, 6201-001 Covilhã, Portugal.
Carbohydr Polym. 2015;127:451-61. doi: 10.1016/j.carbpol.2015.03.089. Epub 2015 Apr 14.
A large group of low molecular weight natural compounds that exhibit antimicrobial activity has been isolated from animals and plants during the past two decades. Among them, peptides are the most widespread resulting in a new generation of antimicrobial agents with higher specific activity. In the present study we have developed a new strategy to obtain antimicrobial wound-dressings based on the incorporation of antimicrobial peptides into polyelectrolyte multilayer films built by the alternate deposition of polycation (chitosan) and polyanion (alginic acid sodium salt) over cotton gauzes. Energy dispersive X ray microanalysis technique was used to determine if antimicrobial peptides penetrated within the films. FTIR analysis was performed to assess the chemical linkages, and antimicrobial assays were performed with two strains: Staphylococcus aureus (Gram-positive bacterium) and Klebsiella pneumonia (Gram-negative bacterium). Results showed that all antimicrobial peptides used in this work have provided a higher antimicrobial effect (in the range of 4 log-6 log reduction) for both microorganisms, in comparison with the controls, and are non-cytotoxic to normal human dermal fibroblasts at the concentrations tested.
在过去的二十年中，人们已经从动植物中分离出了一大类具有抗菌活性的低分子量天然化合物。其中，肽是最广泛存在的，这导致了新一代具有更高特异性的抗菌剂的出现。在本研究中，我们开发了一种新策略，通过将抗菌肽掺入由阳离子（壳聚糖）和阴离子（海藻酸钠盐水）在棉纱布上交替沉积构建的聚电解质多层膜中，获得抗菌伤口敷料。采用能量色散 X 射线微分析技术来确定抗菌肽是否穿透了薄膜。进行了傅立叶变换红外分析以评估化学键合，并且使用两种菌株（金黄色葡萄球菌（革兰氏阳性菌）和肺炎克雷伯菌（革兰氏阴性菌））进行了抗菌试验。结果表明，与对照相比，在本工作中使用的所有抗菌肽均为两种微生物提供了更高的抗菌效果（范围为 4 对数减少至 6 对数减少），并且在测试浓度下对正常的人真皮成纤维细胞无细胞毒性。
