基于透明质酸/壳聚糖/细菌纤维素的膜的细胞毒性评估
Evaluation of Cytotoxicity of Hyaluronic Acid/Chitosan/Bacterial Cellulose-Based Membrane.
作者信息
Dechojarassri Duangkamol, Okada Tomoki, Tamura Hiroshi, Furuike Tetsuya
机构信息
Faculty of Chemistry, Materials and Bioengineering, Kansai University, Osaka 564-8680, Japan.
Organization for Research and Development of Innovative Science and Technology (ORDIST), Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan.
出版信息
Materials (Basel). 2023 Jul 24;16(14):5189. doi: 10.3390/ma16145189.
Novel wound dressing materials are required to non-cytotoxic with a viable cell ratio of above 92%. Herein, the cytotoxicity of hyaluronic acid/chitosan/bacterial cellulose-based (BC(CS/HA)) membranes are evaluated and compared to that of alginate/chitosan/bacterial cellulose-based (BC(CS/Alg)) membranes was investigated. Multilayer membranes with up to ten CS/HA or CS/Alg layers were prepared using the layer-by-layer (LBL) method. Scanning electron microscopy showed that the diameters of the fibers in the BC(CS/Alg) and BC(CS/HA) membranes were larger than those in a BC membrane. The cytotoxicity was analyzed using BALB-3T3 clone A31 cells (mouse fibroblasts, 1 × 10 cells/well). The BC(CS/HA) and BC(CS/HA) membranes exhibited high biocompatibility, with the cell viabilities of 94% and 87% at 5 d, respectively, compared to just 82% for the BC(CS/Alg) and BC(CS/Alg) membranes with same numbers of layers. These results suggested that BC(CS/HA) is a promising material for wound dressings.
新型伤口敷料材料要求无细胞毒性，活细胞比例高于92%。在此，评估了透明质酸/壳聚糖/细菌纤维素基（BC(CS/HA)）膜的细胞毒性，并与藻酸盐/壳聚糖/细菌纤维素基（BC(CS/Alg)）膜的细胞毒性进行了比较。使用层层（LBL）法制备了多达十层CS/HA或CS/Alg的多层膜。扫描电子显微镜显示，BC(CS/Alg)和BC(CS/HA)膜中的纤维直径大于BC膜中的纤维直径。使用BALB-3T3克隆A31细胞（小鼠成纤维细胞，1×10个细胞/孔）分析细胞毒性。BC(CS/HA)和BC(CS/HA)膜表现出高生物相容性，在第5天时细胞活力分别为94%和87%，而相同层数的BC(CS/Alg)和BC(CS/Alg)膜的细胞活力仅为82%。这些结果表明，BC(CS/HA)是一种有前途的伤口敷料材料。
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