Luo H J, Xiao X M, Zhou J, Wei W
Obstetrics and Gynecology Department, The First Affiliated Hospital of Jinan University, Huangpu Road West, Tianhe District, Guangzhou, Guangdong Province, China.
Obstetrics and Gynecology Department, The First Affiliated Hospital of Jinan University, Huangpu Road West, Tianhe District, Guangzhou, Guangdong Province, China
Genet Mol Res. 2015 Apr 17;14(2):3606-17. doi: 10.4238/2015.April.17.10.
To study the effect of Wharton's jelly-derived mesenchymal stem cells (WJMSCs) on the rat salpingitis model, 50 female Wistar rats were randomly divided into one control and five model groups. Mixed bacteria were injected into the oviducts of model groups. The treated acute and chronic groups received intraperitoneal injections of WJMSCs (1 x 10(6)) once a week for three weeks. Serum inflammation factor, collagen fiber content and oviduct-specific glycoprotein (OVGP) levels were detected in control, chronic, ex-treatment acute and chronic, and treated acute and chronic groups (N = 5 for each group). Pregnancy rate and litter size of control, chronic, treated acute and treated chronic groups were compared. Serum TNF-α and INF-γ levels increased in ex-treatment acute and chronic groups, and restored to normal in the acute treated group but not in the treated chronic group. Oviduct collagen fibers did not increase significantly before or after treatment in the acute group, but it increased in the ex-treatment chronic group and did not improve after treatment. After treatment, OVGP levels restored to normal in the acute group but reduced in the ex-treatment and treated chronic groups and chronic group. The pregnancy rate and litter size of the treated acute group recovered to normal, but in the treated chronic group and chronic model group, they decreased significantly. Thus, intraperitoneal injection of WJMSCs could recover the function of the oviduct in acute salpingitis rats, but its effect on chronic salpingitis was poor. Timely treatment of salpingitis is crucial to preserve reproductive function.
为研究沃顿胶源性间充质干细胞（WJMSCs）对大鼠输卵管炎模型的影响，将50只雌性Wistar大鼠随机分为1个对照组和5个模型组。向模型组大鼠输卵管内注射混合菌。治疗的急性和慢性组每周腹腔注射一次WJMSCs（1×10⁶），共注射三周。检测对照组、慢性组、治疗前急性和慢性组以及治疗后急性和慢性组（每组N = 5）的血清炎症因子、胶原纤维含量和输卵管特异性糖蛋白（OVGP）水平。比较对照组、慢性组、治疗后急性组和治疗后慢性组的妊娠率和产仔数。治疗前急性和慢性组血清TNF-α和INF-γ水平升高，治疗后急性组恢复正常，但治疗后慢性组未恢复正常。急性组治疗前后输卵管胶原纤维无明显增加，但治疗前慢性组增加，治疗后未改善。治疗后，急性组OVGP水平恢复正常，但治疗前和治疗后慢性组及慢性组降低。治疗后急性组的妊娠率和产仔数恢复正常，但治疗后慢性组和慢性模型组显著降低。因此，腹腔注射WJMSCs可恢复急性输卵管炎大鼠的输卵管功能，但其对慢性输卵管炎的效果较差。及时治疗输卵管炎对保留生殖功能至关重要。
