The Department of Obstetrics and Gynecology at the 1st Affiliated Hospital of Jinan University, Guangzhou, 510000, China.
The Department of Reproduction at the Southern Medical University Affiliate Dongguan People's Hospital, Dongguan, China.
Stem Cell Res Ther. 2017 Oct 17;8(1):232. doi: 10.1186/s13287-017-0685-0.
The present study was conducted to evaluate new methods to repair the reproductive function of the oviduct, thereby allowing gametes to combine and grow in vivo under natural circumstances.
Sixty pathogen-free female New Zealand rabbits were divided into three groups: a wild-type group, an untreated control group, and a treatment group. Disposable sterile newborn sputum suction tubes were inserted into the urogenital tract to instill an Escherichia coli suspension into the uterine cavity to establish the chronic salpingitis model. Wharton's jelly-derived mesenchymal stem cells (WJMSCs) or normal saline were used to treat this infection via different methods. The therapeutic effect was assessed by evaluating morphology, inflammatory factors, proteinology, and pregnancy outcomes.
Oviducts of New Zealand rabbits in the untreated control group showed structural failure and abnormal supermicrostructure of epithelial cells. WJMSCs could partially repair the structure and supermicrostructure of the tubal epithelium. The concentration of tumor necrosis factor (TNF)-α in the untreated control group was significantly higher than that in the wild-type group (P = 0.015). The concentration of TNF-α in the local treatment group was significantly lower than that in the untreated control group (P = 0.011). The expression of oviductal glycoprotein (OVGP) and OVGP mRNA in the wild-type group was significantly higher than those in the untreated control group (P = 0.024 and P = 0.013, respectively). The litter size of the treatment group was 2 ± 2.39 kits, which was higher than that of the untreated control group (P = 0.035).
Chronic inflammation can destroy the structure of the oviduct and the supermicrostructure of epithelial cells as well as leading to infertility. WJMSC transplantation therapy in rabbits with chronic salpingitis partially restored fertility. WJMSCs also repaired the structure of the tubal epithelium subjected to chronic inflammation, decreased the level of inflammatory factors, and partially restored the secretion level of OVGP.
本研究旨在评估修复输卵管生殖功能的新方法，从而使配子能够在自然环境下结合和生长。
将 60 只无特定病原体的雌性新西兰兔分为三组：野生型组、未治疗对照组和治疗组。将一次性无菌新生儿吸痰管插入生殖道，将大肠杆菌混悬液注入子宫腔，建立慢性输卵管炎模型。用不同方法用牙髓间充质干细胞（WJMSCs）或生理盐水治疗这种感染。通过评估形态、炎症因子、蛋白组学和妊娠结局来评估治疗效果。
未治疗对照组新西兰兔的输卵管结构破坏，上皮细胞超微结构异常。WJMSCs 可部分修复输卵管上皮的结构和超微结构。未治疗对照组肿瘤坏死因子（TNF）-α浓度明显高于野生型组（P=0.015）。局部治疗组 TNF-α浓度明显低于未治疗对照组（P=0.011）。野生型组输卵管糖蛋白（OVGP）和 OVGP mRNA 的表达明显高于未治疗对照组（P=0.024 和 P=0.013）。治疗组的产仔数为 2±2.39 只，明显高于未治疗对照组（P=0.035）。
慢性炎症可破坏输卵管的结构和上皮细胞的超微结构，导致不孕。WJMSC 移植疗法可部分恢复慢性输卵管炎兔的生育能力。WJMSCs 还修复了慢性炎症引起的输卵管上皮结构，降低了炎症因子水平，并部分恢复了 OVGP 的分泌水平。
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