School of Environmental Science and Engineering; Key Laboratory of Urban Agriculture (South), Ministry of Agriculture; School of Agriculture and Biology, and Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai 200240, PR China.
School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, PR China.
Biosens Bioelectron. 2015 Oct 15;72:95-9. doi: 10.1016/j.bios.2015.04.021. Epub 2015 Apr 13.
This paper reports a simple fluorescent assay for the determination of Hg(2+) and Pb(2+) by using a DNA intercalator GeneFinder™ (GF) and an integrated functional nucleic acid (FNA). In the absence of Hg(2+) and Pb(2+), GF intercalated with the FNA and released moderate strong fluorescence. While in the presence of Hg(2+) or Pb(2+), the FNA would be induced to form T-Hg(2+)-T or G-quadruplex structure, interacted with which the GF would exhibit extremely strong or very weak fluorescence. By monitoring the fluorescence changes upon addition of these two ions, the Hg(2+) and Pb(2+) could be selectively detected as low as 3.23 ppb and 2.62 ppb. As the main advantage of this assay is simplicity and the feasibility was demonstrated by detecting Hg(2+) and Pb(2+) in spiked water samples, this assay holds great potential for the development of a cost effective and useful tool for environmental monitoring.
本文报道了一种利用 DNA 嵌入剂 GeneFinder™（GF）和整合功能核酸（FNA）测定 Hg(2+)和 Pb(2+)的简单荧光分析法。在没有 Hg(2+)和 Pb(2+)的情况下，GF 与 FNA 嵌入并释放出中等强度的荧光。而在存在 Hg(2+)或 Pb(2+)的情况下，FNA 会被诱导形成 T-Hg(2+)-T 或 G-四链体结构，与 GF 相互作用会表现出极强或极弱的荧光。通过监测加入这两种离子后荧光的变化，可以选择性地检测到低至 3.23 ppb 和 2.62 ppb 的 Hg(2+)和 Pb(2+)。由于该测定方法的主要优点是简单，并且通过检测加标水样中的 Hg(2+)和 Pb(2+)验证了其可行性，因此该测定方法在开发用于环境监测的经济有效的有用工具方面具有很大的潜力。
